某复方利多卡因乳膏对小鼠局部表面麻醉起效时间的评估

Evaluation of the Onset of Action Time of A Compound Lidocaine Cream on Local Surface Anesthesia in Mice

目的:为了探究某种复方利多卡因乳膏在小鼠表面局部麻醉的起效时间。方法:选用在医学实验中常用的五种品系小鼠(BALA-C/Nude、BALB/C、NOD/SCID、C57BL/6J、C3H),分别在背部涂抹复方利多卡因乳膏,采用模拟接种PDX瘤块于皮下的针刺操作,然后采用计分方式评估小鼠表情变化、针刺躲闪反应、针刺痛觉反应,反应越小评分越低,表明麻醉效果越好。时间点分别为:0、10、20、30、40、50、60 min。结果:各品系小鼠局麻反应有所差异,但是总的趋势为:40 min后,评分普遍降低,表明局麻药品的药效发挥作用。结论:在小鼠进行人源肿瘤异种移植(patient-derived tumor xenograft,PDX)瘤块植入,简单皮肤切开缝合时建议在涂抹复方利多卡因40 min后开始操作,较符合动物福利。

Objective:To investigate the onset time of a compound lidocaine cream on topical anesthesia in mice.Methods:Five strains of mice commonly used in medical experiments(BALA-C/Nude,BALB/C,NOD/SCID,C57BL/6J,C3H)were selected.Compound lidocaine cream was applied on the back respectively,and the acupuncture operation simulated inoculating PDX tumor under the skin was used,and then the expression change,acupuncture evasion response and needle pain response of mice were evaluated by scoring method,and the smaller the response,the lower the score,indicating that the anesthesia effect was better.The time points were:0,10,20,30,40,50,60 min.Results:The response to local anesthesia was different in mice of different strains,but the general trend was as follows:after 40 minutes,the score was generally reduced,indicating that the efficacy of local anesthetic drugs played a role.Conclusion:In mice undergoing PDX tumor mass implantation,it is recommended to start the operation after 40 min after applying compound lidocaine when simple dermatotomy and suture,which is more in line with animal welfare.