DNMT1在口腔鳞状细胞癌中的表达及作用机制研究

Expression and mechanism of DNMT1 in oral squamous cell carcinoma

目的:探讨DNMT1在口腔鳞癌中的表达情况及其对口腔鳞癌细胞增殖、迁移及侵袭的影响。方法:通过GEPIA2数据库分析泛癌中DNMT1表达情况;采用RT-qPCR、Western blot检测人口腔上皮角化细胞HOK及口腔鳞状细胞癌细胞系HSC3、SAS中DNMT1的表达水平;采用shRNA慢病毒敲低HSC3、SAS细胞中DNMT1并用RT-qPCR、Western blot检测sh DNMT1转染效率;采用CCK-8、平板克隆实验检测敲低DNMT1对HSC3、SAS细胞增殖的影响;采用细胞划痕、Transwell实验检测敲低DNMT1对HSC3、SAS细胞迁移、侵袭的影响。结果:与HOK相比,HSC3、SAS细胞中DNMT1的mRNA及蛋白表达量均较高(P<0.05);敲低DNMT1后,HSC3、SAS细胞的增殖、迁移及侵袭能力均降低(P<0.05)。结论:DNMT1在口腔鳞状细胞癌细胞中高表达,且可促进口腔鳞状细胞癌细胞的增殖、迁移和侵袭能力。

Objective:To investigate the expression of DNMT1 in oral squamous cell carcinoma,and to explore its effect on cell proliferation,migration,and invasion.Methods:The GEPIA2 database was used to analyze the expression of DNMT1 in pan-cancers.RT-qPCR and Western Blot were used to detect the expression of DNMT1 in oral epithelial cell lines HOK,HSC3 and SAS.The shRNA lentivirus was used to knock down DNMT1 in HSC3 and SAS cells,and RT-qPCR and Western Blot were used to detect the knockdown efficiency of sh DNMT1.The effects of DNMT1 knockdown on the proliferation of HSC3 and SAS cells were detected by CCK-8 and plate cloning assay,and the effects of DNMT1 knockdown on the migration and invasion of HSC3 and SAS cells were detected by wound healing and Transwell assay.Results:DNMT1 mRNA and protein expressions in HSC3 and SAS cells were higher than in HOK cells(P<0.05).After DNMT1 knockdown,the proliferation,migration and invasion of HSC3 and SAS cells were decreased(P<0.05).Conclusion:DNMT1 is highly expressed in oral squamous cell carcinoma cells and can promote the proliferation,migration and invasion of oral squamous cell carcinoma cells.