真空干燥重组运动发酵单胞菌发酵剂的研制

Development of Vacuum-Dried Recombinant Zymomonas mobilis Fermentation Agent

利用重组运动发酵单胞菌制备乙醇发酵剂,研究保护剂、菌泥与保护剂比例、复合保护剂以及真空干燥条件等因素对菌体存活率的影响。实验结果表明,单一乳糖对重组运动发酵单胞菌的保护效果最佳,菌泥与乳糖的最佳配比为1∶1,菌体存活率为83.24%。分别以乳糖质量的10%、15%、20%添加全蛋粉、蔗糖、海藻糖以及山梨醇组成复合保护剂,得到乳糖与蔗糖复合保护剂的菌体存活率达到最高为92%。通过对干燥过程中复合保护剂与菌泥比例、温度、真空度优化后得到菌体的存活率进一步提高至95.96%。实验结果表明,复合保护剂对重组运动发酵单胞菌在真空干燥条件下具有很好的保护作用。

The experiment was designed to prepare ethanol fermentation agent by using recombinant Zymomonas mobilis.The effect of protective agent,ratio of strain slurry to protective agent,compound protective agent and vacuum drying conditions on the survival rate of the strains were investigated.The experimental results showed that single lactose had the best protective effect on the recombi-nant Zymomonas mobilis,and the optimal ratio of strain slurry to lactose was 1∶1.The survival rate of the strains was 83.24%.10%,15%and 20%whole egg powder,sucrose,alginate and sorbitol were added to the lactose to form a compound protective agent,and the compound protective agent of lactose and sucrose had the highest strain survival rate(92%).The survival rate of the strains was further increased to 95.96%by optimizing the ratio of the compound protective agent to the strain slurry,the temperature and the vac-uum degree during the drying process.The experimental results showed that the compound protective agent had a good protective ef-fect on the recombinant Zymomonas mobilis under vacuum drying conditions.