摘要
【目的】揭示芪蓝复方对妊娠母猪免疫功能的增强作用,为缓解妊娠母猪免疫抑制提供参考,为芪蓝复方的临床应用提供数据支撑。【方法】采用加热回流、浓缩冻干工艺制备芪蓝复方提取物,利用硫酸-苯酚法、高效液相色谱法和紫外分光光度法检测芪蓝复方的主要有效成分含量。选取25头长白二元母猪(30~40胎龄),分为空白组(N),0.1%(L)、0.2%(M)和0.4%(H)芪蓝复方组以及阳性对照组(A),5头/组,试验期30 d,分别于试验开始后1、15和29 d采集血液,检测血清生化指标(总蛋白、白蛋白、尿素氮、葡萄糖、血钙、血磷和甘油三酯)、血清抗氧化指标(总抗氧化能力、谷胱甘肽过氧化物酶、超氧化物歧化酶和丙二醛)、免疫球蛋白(IgG、IgM和IgA)、炎症因子(白细胞介素-6(IL-6)和IL-1β)以及猪繁殖与呼吸综合征病毒(PRRSV)抗体水平。【结果】芪蓝复方中多糖、(R,S)-告依春以及淫羊藿苷含量分别为56.0%、0.035%和0.39%。妊娠15、29 d,与N组相比,A组猪血清白蛋白、IgM、IgA含量及总抗氧化能力、谷胱甘肽过氧化物酶活性均显著升高(P<0.05);L、H组谷胱甘肽过氧化物酶和超氧化物歧化酶活性均显著升高(P<0.05);各组丙二醛、IL-1β含量均显著降低(P<0.05);M、H组S/P值均显著降低(P<0.05)。与A组相比,妊娠15 d, L组血清总蛋白、血钙水平、总抗氧化能力及IgM、IgA含量均显著降低(P<0.05);H组超氧化物歧化酶活性显著升高,血清总蛋白、血钙、甘油三酯水平及IgM、IL-6含量、S/P值均显著降低(P<0.05)。妊娠29 d, L组血清总抗氧化能力、血钙水平、IgG、IgA含量均显著降低(P<0.05);M组血钙水平、超氧化物歧化酶活性、IL-1β含量均显著降低(P<0.05)。【结论】芪蓝复方对妊娠母猪免疫功能具有显著增强作用,可用于妊娠母猪免疫抑制疾病防控。
【Objective】This experiment was aimed to reveal the effect of Qilan compound on immune function of pregnant sows,provide reference for relieving immune suppression of pregnant sows,and provide data support for clinical application of Qilan compound.【Method】The extract of Qilan compound was prepared by reflux heating and lyophilization,and its main active components were detected by phenol-sulfuric acid method,HPLC and UV spectrophotometry.25 Landrace binary sows(30-40 gestural age)were selected and divided 5 groups:Negative control group(group N),0.1%,0.2%and 0.4%Qilan compound groups(groups L,M and H)and positive control group(group A),5 sows in each group.The experiment lasted for 30 days,and blood samples were collected on 1,15 and 29 days.Serum biochemical indices(total protein,albumin,urea nitrogen,glucose,calcium,phosphorus and triglyceride),serum antioxidant function(total antioxidant capacity,glutathione peroxidase,superoxide dismutase and malondialdehyde),immunoglobulin(IgG,IgM and IgA),inflammatory factors(IL-6 and IL-1β)and PRRSV antibody levels were determined.【Result】The contents of polysaccharide,epigoitrin and icariin in Qilan compound were 56.0%,0.035%and 0.39%,respectively.On days 15 and 29 of gestation,compared with group N,the contents of serum albumin,IgM and IgA,total antioxidant capacity and glutathione peroxidase activity in group A were significantly increased(P<0.05).The activities of glutathione peroxidase and superoxide dismutase in groups L and H were significantly increased(P<0.05).The contents of malondialdehyde and IL-1βwere significantly decreased in all groups(P<0.05).The S/P values in groups M and H were significantly decreased(P<0.05).Compared with group A,on day 15 of gestation,the levels of serum total protein,calcium,total antioxidant capacity and IgM and IgA contents in group L were significantly decreased(P<0.05).The activity of superoxide dismutase in group H was significantly increased,and the levels of serum total protein,calcium,triglyceride,IgM,IL-6 contents and S/P value were significantly decreased(P<0.05).On day 29 of gestation,serum total antioxidant capacity,serum calcium level and IgG,IgA contents in group L were significantly decreased(P<0.05).Serum calcium level,superoxide dismutase activity and IL-1βcontent in group M were significantly decreased(P<0.05).【Conclusion】Qilan compound could significantly promote the immune function of pregnant sows,and it could be used for the prevention and control of immunosuppressive disease of pregnant sows.
作者
杨黎宇
李燕玲
范舒钰
李健
YANG Liyu;LI Yanling;FAN Shuyu;LI Jian(Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health,Fujian Agriculture and Forestry University,Fuzhou 350002,China;University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province,Fuzhou 350002,China)
出处
《中国畜牧兽医》
CSCD
北大核心
2024年第1期64-73,共10页
China Animal Husbandry & Veterinary Medicine
基金
福建省高校产学合作项目(2019N5004)
福建省重大专项专题项目(2021NZ029008)。
关键词
芪蓝复方
妊娠母猪
免疫功能
免疫抑制
Qilan compound
pregnant sows
immune function
immunosuppression