3种中药成分对玉米赤霉烯酮致小鼠肝脏损伤的缓解作用

Alleviative Effects of Three Traditional Chinese Medicine Components on Liver Injury in Zearalenone Poisoned Mice

【目的】探究中药对玉米赤霉烯酮(zearalenone, ZEA)致动物肝损伤的缓解作用。为临床研发新中兽药和饲料添加剂提供理论依据。【方法】将63只8周龄昆明雌鼠随机分为7组,每组9只。对照组,上、下午灌胃生理盐水各1次;ZEA组,上午灌胃20 mg/kg ZEA,下午灌胃生理盐水;槲皮素、山奈酚、芦丁和成分复方组为中药成分治疗组,上午灌胃20 mg/kg ZEA,下午分别灌胃50 mg/kg槲皮素、50 mg/kg山奈酚、100 mg/kg芦丁、100 mg/kg成分复方(V_((槲皮素))∶V_((山奈酚))∶V_((芦丁))=1∶2∶2);阳性对照组,上午灌胃20 mg/kg ZEA,下午灌胃100 mg/kg维生素E;每只每次均灌胃0.2 mL,连续7 d。于第8天称量各组小鼠体重,眼球摘除采血,分离血清,脱颈处死小鼠,剥离肝脏并称重,计算肝脏指数,检测血清肝脏功能、抗氧化酶活性和肝脏炎症指标。【结果】生长发育检测结果显示,与对照组相比,ZEA组小鼠体重降低、肝脏重量升高(P<0.05);与ZEA组相比,芦丁和成分复方组体重升高(P<0.05)。血清肝脏功能检测结果,与对照组相比,ZEA组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)活性均升高(P<0.05);与ZEA组相比,中药成分和维生素E治疗组各个酶活性均降低(P<0.05)。肝脏氧化应激检测结果显示,与对照组相比,ZEA组肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量和总抗氧化能力(T-AOC)均降低(P<0.05),丙二醛(MDA)和LDH含量升高(P<0.05);与ZEA组相比,中药成分和维生素E治疗组SOD、CAT活性、GSH含量和T-AOC均升高(P<0.05),MDA和LDH含量降低(P<0.05)。炎症因子检测结果,与对照组相比,ZEA组机体肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)和IL-6含量升高、IL-10含量降低(P<0.05);与ZEA组相比,中药成分和维生素E治疗组机体TNF-α、IL-1β和IL-6含量降低(P<0.05),槲皮素和维生素E治疗组肝脏IL-10含量升高(P<0.05)。【结论】3种中药成分槲皮素、山奈酚、芦丁和维生素E均可缓解ZEA致小鼠肝脏的损伤,山奈酚效果最佳。

【Objective】This study was aimed to explore the relieving effect of traditional Chinese medicine on liver damage caused by zearalenone(ZEA)in animals.【Method】638-week-old Kunming female mice were randomly divided into 7 groups,with 9 mice in each group.In control group,physiological saline was administered orally once in the morning and once in the afternoon;ZEA group,20 mg/kg ZEA was gavaged in the morning and physiological saline was gavaged in the afternoon.Quercetin,kaempferol,rutin,and compound ingredients groups were traditional Chinese medicine treatment groups.In the morning,20 mg/kg ZEA was administered by gavage,and in the afternoon,50 mg/kg quercetin,50 mg/kg kaempferol,100 mg/kg rutin,and 100 mg/kg compound ingredients(V_((quercetin))∶V_((kaempferol))∶V_((rutin))=1∶2∶2)were administered by gavage,respectively.Positive control group,20 mg/kg ZEA were administered by gavage in the morning and 100 mg/kg vitamin E was administered by gavage in the afternoon.Each animal was gavaged with 0.2 mL each time for 7 consecutive days.The mice were euthanized on the 8th day to detect body weight,liver index,serum liver function,antioxidant enzyme activity,and liver inflammation indicators.【Result】The weight development results showed that compared with control group,ZEA group mice had lower body weight and higher liver weight(P<0.05).Compared with ZEA group,the weight of rutin and compound groups were increased(P<0.05).Compared with control group,the serum liver function test results showed that the activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),acid phosphatase(ACP),lactate dehydrogenase(LDH)in ZEA group were increased(P<0.05).Compared with ZEA group,the activity of various enzymes in traditional Chinese medicine and vitamin E treatment groups were decreased(P<0.05).The results of liver oxidative stress test showed that compared with control group,the activity of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH)content,and total antioxidant capacity(T-AOC)in liver of ZEA group were decreased(P<0.05),while the content of malondialdehyde(MDA)and LDH were increased(P<0.05).Compared with ZEA group,the activity of SOD,CAT,GSH content and T-AOC in traditional Chinese medicine and vitamin E treatment groups were increased(P<0.05),while the content of MDA and LDH were decreased(P<0.05).Inflammatory factor detection results showed that compared with control group,the content of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 in ZEA group were increased and the content of IL-10 was decreased(P<0.05).Compared with ZEA group,TNF-α,IL-1βand IL-6 contents of traditional Chinese medicine ingredient and vitamin E treatment groups were decreased(P<0.05),while the IL-10 content were increased in quercetin and vitamin E treatment groups(P<0.05).【Conclusion】All three traditional Chinese medicine ingredients quercetin,kaempferol,rutin and vitamin E could alleviate the liver damage caused by ZEA in mice,and the effect of kaempferol was the best.