CFH蛋白在家系早发冠心病血瘀证中的表达及意义

Expression and significance of CFH protein in familial premature coronary heart disease with blood stasis pattern

目的筛选家系早发冠心病血瘀证的特异性蛋白,为今后临床早期诊断和预防疾病提供潜在生物标志物。方法通过同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantification,i TRAQ)分析家系早发冠心病血瘀证患者、家系早发冠心病非血瘀证患者及健康人的血浆蛋白表达,数据分析后得到各组间的差异蛋白表达情况,再经差异蛋白筛选标准初步得到疾病的预测蛋白,并使用Western blot验证预测蛋白。结果通过i TRAQ技术共得到差异蛋白75个,其中家系早发冠心病血瘀证组与健康对照组之间共得到32个差异蛋白,包括22个上调蛋白与10个下调蛋白,共涉及429个生物学过程,其与角质化、皮肤发展、蛋白质激活级联反应等关系最为密切;在代谢途径金黄色葡萄球菌感染、补体和凝血级联、血小板激活等KEGG通路上富集。与健康对照组相比,家系早发冠心病血瘀证组差异蛋白补体因子H(complement factor H,CFH)表达水平差异有统计学意义(P<0.01)。结论经差异蛋白筛选标准得到的CFH蛋白,可作为家系早发冠心病血瘀证早期诊断的潜在生物标志物。

Objective To screen the specific proteins of familial premature coronary heart disease(PCHD)with blood stasis pattern,and to provide potential biomarkers for early clinical diagnosis and prevention of the disease in the future.Methods The plasma protein expressions of familial PCHD patients with blood stasis pattern,familial PCHD patients without blood stasis pattern,and healthy control groups were analyzed by isobaric tags for relative and absolute quantification(iTRAQ).After data analysis,the differential protein expressions among the groups were obtained to preliminarily get the predicted protein of the disease by the differential protein screening criteria.The predicted protein was verified by Western blot.Results A total of 75 differential proteins were obtained by iTRAQ,of which 32 differential proteins were obtained between the familial PCHD with blood stasis pattern group and the healthy control group,including 22 up-regulated proteins and 10 down-regulated proteins,involving 429 biological processes,which were most closely related to keratinization,skin development,and protein activation cascade reaction;they were enriched in KEGG pathways such as Staphylococcus aureus infection,complement and coagulation cascades,and platelet activation.Compared with the healthy control group,the difference of the expression level of complement factor H(CFH)protein in the familial PCHD with blood stasis pattern group had statistical significance(P<0.01).Conclusion The CFH protein obtained by the differential protein screening criteria can be used as a potential biomarker for the early diagnosis of familial PCHD with blood stasis pattern.