新疆布尔根河狸国家级自然保护区狭耳鼠耳蝠线粒体基因组研究

Study on the Mitochondrial Genomes of Myotis blythii in the Buergen Beaver National Nature Reserve,Xinjiang

为提供鼠耳蝠属(Myotis)蝙蝠类遗传及进化分子水平的证据,通过高通量测序法对新疆布尔根河狸国家级自然保护区栖息的狭耳鼠耳蝠(M.blythii)线粒体基因组全序列进行测定和分析。结果显示:狭耳鼠耳蝠线粒体基因组总长为16752 bp,总碱基组成为33.8%A、30.4%T、22.7%C和13.1%G;线粒体基因组由13个蛋白质编码基因、2个r RNA(12S和16S)基因、22个t RNA基因和1个非编码控制区组成;控制区基因长度为948 bp,比大鼠耳蝠(M.myotis)等姊妹物种短很多;与从NCBI下载的其他鼠耳蝠属蝙蝠线粒体基因组序列的系统发育分析比较,狭耳鼠耳蝠与大鼠耳蝠亲缘关系最近。研究结果丰富了鼠耳蝠属线粒体基因组信息,明确了新疆狭耳鼠耳蝠在鼠耳蝠属内的系统进化地位和亲缘关系,为蝙蝠科(Vespertilionidae)系统分子进化和后续研究提供基础数据。

To provide molecular level evidence for the genetics and evolution of bats belonging to the genus Myotis,this study used high-throughput sequencing to determine and analyze the mitochondrial genome sequence of M.blythii,a species inhabiting at the Buergen Beaver National Nature Reserve of Xinjiang,China.Results showed that the total length of the mitochondrial genome of M.blythii was 16,752 bp,and the total base groups were 33.8% A,30.4% T,22.7% C,and 13.1% G.The mitochondrial genome consisted of 13 protein coding genes,2 rRNAs(12S and 16S RNA) genes,22 tRNA genes,and a noncoding control region.The length of the control region gene was 948 bp,which was much shorter than that of sister species such as M.myotis.Based upon mitochondrial genome sequences collected from NCBI for phylogenetic analysis of other bat species belonging to the genus Myotis,the analysis showed that M.blythii and M.myotis had the closest genetic relationship.The research findings of this study have enriched the mitochondrial genome information of this species,clarified the phylogenetic status and phylogenetic relationship of M.blythii in Xinjiang within the genus Myotis,and provided a data basis for the systematic and molecular evolution of Vespertilionidae and subsequent research.