加味七方胃痛颗粒含药血清抑制人胃癌AGS细胞铁死亡的机制研究

Study on the mechanism of inhibiting iron death of AGS cells in Jiawei Qifang Weitong Granules

目的 探讨加味七方胃痛颗粒抑制胃癌细胞铁死亡效应机制。方法 选取Wistar大鼠制备含药血清;胃癌AGS细胞分为AGS组、空白血清组和含药血清组。含药血清组大鼠以19.4 g/(kg·d)的换算方法灌胃加味七方胃痛颗粒7 d制备含药血清。空白血清组大鼠常规喂养制备空白血清。干预2 d后检测各组细胞活性和形态,比色法检测丙二醛(MDA)、谷胱甘肽(GSH)含量,RT-q PCR、Western blot检测SLC7A11、P53、GPX4 mRNA含量及蛋白表达水平。结果 含药血清组细胞增殖率低于空白血清组(P<0.05)。含药血清组SLC7A11、GPX4 mRNA的含量低于空白血清组,P53 mRNA含量高于空白血清组(P<0.01)。含药血清组SLC7A11、GPX4的蛋白表达水平低于空白血清组,P53的蛋白表达水平高于空白血清组(P<0.01)。含药血清组MDA含量低于空白血清组,GSH含量高于空白血清组(P<0.01)。结论 七方胃痛颗粒可抑制胃癌细胞增殖,其效应可能与抑制细胞铁死亡信号有关。

Objective To investigate the mechanism of Jiawei Qifang Weitong Granules on inhibiting iron death of in gastric cancer cells.Methods Wistar rats were selected to prepare drug-containing serum.Gastric cancer AGS cells were divided into AGS group,blank serum group,and drug-containing serum group.The drug-containing serum was prepared by the conversion method of 19.4 g/(kg·d)intragastric administration of supplemented Qifang Weitong Granules fors seven days.Blank serum was prepared by routine feeding of rats in blank serum group.After two days of intervention,the cell activity and morphology of each group were detected,the contents of malondialdehyde(MDA)and glutathione(GSH)were detected by colorimetry,and the mRNA contents and protein expression levels of SLC7A11,P53 and GPX4 were detected by RT-qPCR and Western blot.Results The cell proliferation rate of drug-containing serum group was lower than that of blank serum group(P<0.05).The mRNA contents of SLC7A11 and GPX4 in drug-containing serum group were lower than those in blank serum group,and the mRNA contents of P53 were higher than those in blank serum group(P<0.01).The expression levels of SLC7A11 and GPX4 in drug-containing serum group were lower than those in blank serum group,and the expression level of P53 was higher than that in blank serum group(P<0.01).The MDA content in drug-containing serum group was lower than that in blank serum group,and the GSH content was higher than that in blank serum group(P<0.01).Conclusion Jiawei Qifang Weitong Granules can inhibit the proliferation of gastric cancer cells,and its effect may be related to the inhibition of ferroptosis signal.