拉曼光谱在细胞成像中的研究进展

Research Progress of Raman Spectroscopy for Cell Imaging

细胞成像在生命科学与药物研究中具有重要意义。拉曼光谱作为一种非破坏性的振动光谱技术,结合非标记或探针标记技术可对细胞不同组分进行成像。由于自发拉曼光谱信号较弱,运用有效的增强手段可提高细胞成像的时间与空间分辨率。该文综述了表面增强拉曼光谱(SERS)、相干拉曼光谱(CRS)、共振拉曼光谱(RRS)等拉曼增强方法在线粒体、溶酶体和内质网等细胞器成像中的研究进展,以及上述方法在蛋白质、脂质、糖类和核酸等重要细胞生物分子成像中的应用。此外,还讨论了标记技术中拉曼探针的化学结构、增强因子、检出限等因素对细胞成像的影响,并分析了当前细胞拉曼成像的发展趋势、存在的挑战和可能的解决方案。

Cell imaging plays a crucial role in life science and drug discovery research.Raman spec-troscopy,a non-destructive vibration spectroscopic technology,enables the imaging of various cellu-lar components when combining with label-free or labeling techniques.Due to the low sensitivity of spontaneous Raman scattering,it is necessary to utilize efficient enhancement methods to increase the space and time resolutions of cellular Raman imaging.In this review,we summarized the ad-vancement of surface-enhanced Raman spectroscopy(SERS),coherent Raman spectroscopy(CRS),resonance Raman spectroscopy(RRS),and other Raman enhancement methods applied for cellular organelle imaging of mitochondria,lysosomes,endoplasmic reticulum,and etc.,as well as for cel-lular biomolecule imaging of proteins,lipids,sugars,nucleic acids and etc.We discussed the fac-tors of labeling techniques influencing cell imaging results,including the probe structures,enhance-ment factors,and detection limits,and analyzed the current challenges and future trends of developing Raman spectroscopic technology for cell imaging.