线粒体活性氧靶向成像荧光探针概述

Overview of Fluorescent Probes for Targeted Imaging of Mitochon⁃drial Reactive Oxygen Species

线粒体作为细胞的能量工厂,在调节细胞氧化还原水平、自由基稳态等方面发挥着重要作用,因此,对线粒体微环境中的活性氧(Reactive oxygen species,ROS)进行监测能加深对线粒体的了解。而化学小分子荧光探针在检测细胞微环境方面具有独特的优势,开发化学小分子靶向细胞线粒体并检测其细胞内微环境的变化对于了解与线粒体相关疾病具有重要意义。该文基于靶向线粒体ROS探针的反应机理,对超氧化阴离子(O2·-)、次氯酸(HClO)、过氧化氢(H2O2)以及一氧化氮(NO)和过氧亚硝基阴离子(ONOO-)等ROS荧光探针进行了介绍,分析了其反应特点、识别机理及不足之处,并展望了线粒体ROS探针在生命医学中的重要意义和发展前景。

Organelles,which are important devices for maintaining normal cellular activity,are specific organelle microenvironments surrounded by lipid bilayers.Different organelles need to per-form their specific cellular functions under different microenvironmental conditions,and the disrup-tion of microenvironmental homeostasis would lead to disease.Among them,mitochondria,as the energy factories of cells,plays an important role in regulating cellular redox levels and free radical homeostasis.Therefore,the monitoring of reactive oxygen species(ROS)in mitochondrial microenvi-ronment can deepen the understanding of the physiopathology associated with it.It has been reported that chemical small molecule fluorescent probes have unique advantages in detecting the cellular mi-croenvironment,and the understanding of mitochondria-associated diseases.Therefore,this paper mainly focus on fluorescent probes targeting ROS such as superoxide anion(O2·-),hypochlorous acid(HClO),hydrogen peroxide(H2O2),and nitric oxide(NO)and peroxynitrite(ONOO-)in mitochondria.