我国多地区献浆者总蛋白水平调查

Total protein level of plasma donors in various regions of China:a survey of 1373 cases

目的分析捐献血浆对人体总蛋白的影响以及不同采血管对总蛋白水平检测的影响。方法选取2021年3—4月来自6个省/自治区共11家单采血浆站1373名献浆者,分别用不加抗凝剂普通采血管、肝素抗凝管、枸橼酸钠抗凝管采集全血标本并分为血清组、肝素抗凝组、枸橼酸钠抗凝组。标本在分离出血清和血浆后用双缩脲法进行总蛋白检测。不同采血管之间总蛋白水平比较采用Kruskal-Wallis检验。按性别将献浆者分为男性组(n=597)、女性组(n=776)2组,不同性别之间总蛋白水平比较采用独立样本t检验。按地域将献浆者分为四川组、湖北组、甘肃组,采用Games-Howell检验,进行两两比较。结果1373名献浆者血清总蛋白水平中位数为73.1 g/L,与我国血清总蛋白参考值范围(65~85)g/L相符合。1373名献血者血清组、肝素抗凝组、枸橼酸钠抗凝组总蛋白水平的中位数分别为:73.1 g/L、73.3 g/L、63.8 g/L,3组间比较有差异(P<0.05)。枸橼酸钠抗凝组与血清组和肝素抗凝组总蛋白水平比较有差异(P<0.05),血清组与肝素抗凝组总蛋白水平比较无差异(P>0.05)。男性组与女性组血清总蛋白水平分别为:(72.41±5.40)g/L、(73.67±4.95)g/L(P<0.05)。四川组、湖北组、甘肃组血清总蛋白水平分别为(73.91±4.29)g/L、(74.17±5.11)g/L、(67.09±3.65)g/L(P<0.05),3组间两两比较,甘肃组与湖北组和四川组有差异(P<0.05),四川组与湖北组无差异(P>0.05)。结论符合献浆条件的献浆者不会由于定期捐献血浆导致体内总蛋白含量异常,但不同性别、不同地域献血者总蛋白水平均存在差异,女性总蛋白水平高于男性,湖北地区总蛋白水平最高,其次四川,最后甘肃地区。不同采血管的总蛋白水平有差异,肝素抗凝组最高,其次血清组,最后枸橼酸钠抗凝组。

Objective To analyze the influence of plasma donation on human total protein level and the impact of different blood collection tubes on total protein level detection.Methods A total of 1373 plasma donors from 11 apheresis plasma stations in 6 provinces/autonomous regions from March to April,2021 were selected.Whole blood was collected by ordinary blood collection tube without anticoagulant,heparin anticoagulant tube and sodium citrate anticoagulant tube,and then respectively divided into serum group,heparin anticoagulant group,and sodium citrate anticoagulant group.After separating serum and plasma,the samples were subjected to total protein detection using the biuret method.Kruskal-Wallis test was used to compare the total protein levels among different tubes.The plasma donors were divided into male group(n=597)and female group(n=776),and the total protein levels between different genders were compared by t test.The plasma donors were divided into Sichuan group,Hubei group and Gansu group according to the region,and the Games-Howell test was used for comparison.Results The median serum total protein level of 1373 donors was 73.1g/L,which was consistent with the reference range of 65-85 g/L.The median total protein levels of the serum group,heparin anticoagulant group and sodium citrate anticoagulant group were 73.1g/L,73.3g/L and 63.8g/L,respectively,with statistically significant difference(P<0.05).There was statistical significance in total protein level between sodium citrate anticoagulant group and serum group,sodium citrate anticoagulant group and heparin anticoagulant group(P<0.05),but no statistical significance was noticed between serum group and heparin anticoagulant group(P>0.05).The serum total protein levels of male group and female group were(72.41±5.40)g/L and(73.67±4.95)g/L,reseectively,and the difference was statistically significant(P<0.05).The serum total protein level in Sichuan group,Hubei group and Gansu group was(73.91±4.29)g/L,(74.17±5.11)g/L and(67.09±3.65)g/L,respectively(P<0.05).The difference between Gansu group and Hubei group,Gansu group and Sichuan group was statistically significant(P<0.05),but no significant difference was noticed between Sichuan group and Hubei group(P>0.05).Conclusion Plasma donors who meet the donation criteria will not experience abnormal total protein levels due to regular plasma donation.There were differences in total protein levels among different blood collection tubes,different genders and different regions.The total protein level of females was higher than that of males.The total protein level was the highest in Hubei province,followed by Sichuan and Gansu.Heparin anticoagulant group was the highest,followed by serum group and sodium citrate anticoagulant group.