子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白的表达及其意义

Expression and significance of N-WASP in placentas with preeclampsia

目的研究子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白(N-WASP)的表达情况及其临床意义。方法选取2020年7月至2021年12月于中国医科大学附属盛京医院分娩的65例孕产妇作为研究对象,并将其分为早发型子痫前期组(15例)、早发对照组(15例)、晚发型子痫前期组(15例)、晚发对照组(20例)。采用实时定量PCR(RT-qPCR)检测各组胎盘组织中N-WASP mRNA的相对表达量,采用Western blotting和免疫组织化学法检测各组胎盘组织中N-WASP蛋白的表达情况及定位情况。结果RT-q PCR、Western blotting结果显示,早发型子痫前期组胎盘组织N-WASP mRNA和蛋白表达水平显著低于早发对照组(0.50±0.19 vs.0.93±0.73,0.35±0.17 vs.0.72±0.21),晚发型子痫前期组胎盘组织中N-WASP mRNA和蛋白表达水平显著低于晚发对照组(0.83±0.34 vs.1.15±0.34,0.39±0.16 vs.0.76±0.20),差异均有统计学意义(均P<0.05)。N-WASP mRNA在胎盘组织中的表达与早发型及晚发型子痫前期的发生均呈负相关(r=-0.37,P=0.042;r=-0.39,P=0.019)。免疫组织化学检测显示N-WASP蛋白定位于合体滋养细胞、细胞滋养细胞、绒毛间质细胞和血管内皮细胞的胞质中。结论N-WASP低表达可能与子痫前期的发生密切相关。

Objective To study the expression and clinical significance of neural Wiskott-Alrdich syndrome protein(N-WASP)in placentas with preeclampsia.Methods This study included a total of 65 pregnant women:15 in the early-onset preeclampsia group,15 in the early-onset control group,15 in the late-onset preeclampsia group,and 20 in the late-onset control group.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the relative expression of N-WASP mRNA in placental tissues.Western blotting and immunohistochemistry were used to detect the expression and position of N-WASP protein in placental tissues from each group.Results RT-qPCR revealed significantly lower N-WASP mRNA expression levels in the placental tissue of the early-onset preeclampsia group compared to those in the early-onset control group(0.50±0.19 vs.0.93±0.73,P<0.05).The N-WASP mRNA expression levels in late-onset preeclampsia placenta were significantly lower than those in the late-onset control group(0.83±0.34 vs.1.15±0.34,P<0.05).Western blotting revealed significantly lower N-WASP protein expression in the placental tissue of early-onset preeclampsia compared to that in the early-onset control group(0.35±0.17 vs.0.72±0.21,P<0.05).The N-WASP protein expression in late-onset preeclampsia placenta was significantly lower than that in the late-onset control group(0.39±0.16 vs.0.76±0.20,P<0.05).The N-WASP mRNA expression in the placenta negatively correlated with the occurrence of early-onset(r=-0.37,P=0.042)and late-onset preeclampsia(r=-0.39,P=0.019).Immunohistochemistry revealed that N-WASP protein was localized in the cytoplasm of syncytiotrophoblasts,cytotrophoblasts,villous stromal cells,and vascular endothelial cells.Conclusion The low expression of N-WASP may be closely associated with preeclampsia.