高温胁迫对‘索邦’百合花色合成的影响

Effects of high temperature on color synthesis of flowers in oriental hybrid lily‘Sorbonne’

为精准探究高温胁迫对百合开花品质的影响,以东方百合‘索邦’为试验材料,研究了不同发育阶段的花苞经过不同温度和不同时间处理后,盛花期花径、花色和矢车菊素含量的变化,以及温度处理过程中矢车菊素合成通路的3个关键基因LhCHS、LhF3H和LhANS的表达量变化。结果显示:S5-S6时期是‘索邦’百合花苞显色的关键时期;花苞在高于30℃温度培养4 d后,盛花期花径显著变小,矢车菊含量显著降低,花被片颜色变浅;qRT-PCR结果显示,花苞显色的关键时期,遇到30℃以上的高温处理导致LhCHS、LhF3H、LhANS等花青素合成基因表达量显著下调,从而影响盛花期花色的合成。

The oriental hybrid lily‘Sorbonne’was used to precisely study the effects of high temperature on the flowering quality of lily.The changes in flower diameter,color,and content of cyanidin at different developmental stages of flower buds treated at different temperatures and times during the period of full bloom were investigated.The changes in the expression level of three key genes involved in the synthesis pathway of cyanidin including LhCHS,LhF3H and LhANS during temperature treatment were identified as well.The results showed that the S5 and S6 stages were key period for color development of'Sorbonne'buds.The flower diameter was significantly smaller,the content of cyaindin was significantly reduced,and the color of tepal was lighter at the stage of full bloom after the buds were treated at a temperature higher than 30℃for 4 days.The results of qRT-PCR analysis showed that high temperature treatment above 30℃during the critical period of flower bud coloration significantly downregulated the expression of anthocyanin synthesis genes such as LhCHS,LhF3H,and LhANS,thereby affecting the synthesis of flower color during the period of full bloom.It will provide a theoretical basis for temperature control in the production and postharvest process of lily‘Sorbonne’.