小桃儿七化学成分研究

Chemical constituents of Helleborus thibetanus

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和半制备高效液相色谱等多种方法对小桃儿七的化学成分进行分离纯化,并运用NMR和MS等现代波谱技术鉴定化合物结构,采用MTT法测定化合物1~8的细胞毒性。结果从小桃儿七中分离得到12个化合物,分别鉴定为(25R)-22β,25-expoxy-26-[(O-β-D-glucopyranosyl)oxy]-1β,3β-dihydroxyfurosta-5-en(1)、β-sitosterol myristate(2)、β-sitosterol lactate(3)、β-sitosterol 3-O-β-D-glucopyrannoside(4)、4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one(5)、间苯三酚三甲醚(6)、7,8-二甲苯基蝶啶-2,4(1H,3H)-二酮(7)、1H-吲哚-3-羧酸(8)、对羟基桂皮酸(9)、十二烷酸(10)、n-butylα-L-arabinofuranoside(11)、甲基-α-D-呋喃果糖苷(12)。其中,化合物1为新化合物,命名为thibetanoside L。化合物2、5~8、11为首次从该科植物中分离得到,化合物12为首次从该属植物中分离得到。MTT法结果表明化合物1~8对人肝癌HepG2细胞和人结肠癌HCT116细胞的IC50均大于100μmol·L^(-1)。

The chemical constituents of Helleborus thibetanus were isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography,and semi-preparative RP-HPLC,and the structures of all compounds were identified by modern spectrographic technology(MS,NMR).The MTT method was used to measure the cytotoxicity of compounds 1-8.Twelve compounds were isolated from the roots and rhizomes of H.thibetanus and were identified as(25R)-22β,25-expoxy-26-[(O-β-Dglucopyranosyl)oxy]-1β,3β-dihydroxyfurosta-5-en(1),β-sitosterol myristate(2),β-sitosterol lactate(3),β-sitosterol 3-O-β-Dglucopyrannoside(4),4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one(5),1,3,5-trimethoxybenzene(6),7,8-dimethylbenzo pteridine-2,4(1H,3H)-dione(7),1H-indole-3-carboxylic acid(8),p-hydroxy cinnamic acid(9),lauric acid(10),n-butylα-Larabinofuranoside(11)and methyl-α-D-fructofuranoside(12),respectively.Among them,compound 1 is a new compound and named thibetanoside L;compounds 2,5-8,11 are first isolated from the family Ranunculaceae;compound 12 is isolated from the genus Helleborus for the first time.The results of MTT assay showed that the IC50 values of compounds 1-8 against HepG2 and HCT116 cells were greater than 100μmol·L-1.