国产离子色谱-自研脉冲安培检测器测定饮料中常见糖类化合物的含量

Determination of Common Carbohydrate Compounds in Beverages by Domestic Ion Chromatography with Self-Designed Pulsed Amperometric Detector

使用国产离子色谱-自研脉冲安培检测器测定饮料中常见的葡萄糖、果糖、乳糖、蔗糖和麦芽糖的含量。结果表明,5种糖类化合物的质量浓度在0.2~5.0 mg·L^(-1)内与对应的峰面积呈线性关系,检出限(3S/N)分别为3.420,6.759,10.12,20.43,31.37μg·L^(-1)。按照标准加入法进行回收试验,回收率为94.1%~114%,测定值的相对标准偏差(n=5)均小于5.0%,说明方法符合相关检测标准要求。为考察国产仪器分析的准确性和评价主要模块的性能,与Thermo ICS 5000+型离子色谱安培检测系统和Dionex Ultimate 3000型液相色谱示差检测器系统进行比较,结果表明,三者的分析结果一致性良好,其中国产离子色谱-脉冲安培检测器系统的检出限比Thermo ICS 5000+离子色谱安培检测系统高3~4倍。除此之外,国产离子色谱仪各个模块性能稳定,可满足常规糖类化合物含量的测定。

Glucose,fructose,lactose.sucrose and maltose commonly found in beverages were determined by domestic ion chromatography with self-designed pulsed amperometric detector.As shown by the results,linear relationship between values of the corresponding peak area and mass concentration of 5 carbohydrate compounds were found in the range of 0.2-5.0 mg·L^(-1),with detection limits(3S/N)of 3.420,6.759,10.12.20.43,31.37μg L^(-1),respectively.Test for recovery was made by the standard addition method,giving the results in the range of 94.1%^(-1)14%,with RSDs(n=5)of the determined values less than 5.0%,indicating that this method could meet the requirements of relevant testing standards.To investigate the accuracy of domestic instrument analysis and evaluate the performance of main modules,it was compared with Thermo ICS 50oo+ion chromatograph amperometric detection system and Dionex Ultimate 3ooo liquid chromatograph differential detector system,and it was shown that the analysis results were consistent,but the detection limit of domestic ion chromatography system with self-designed pulsed amperometric detector was 3 to 4 times higher than the imported instrument.The performance of each module and machine of domestic ion chromatography instrument was stable,and it could meet the requirements for the determination of conventional carbohydrate compounds.