染色质免疫共沉淀测序技术及其在园艺植物中的应用研究进展

Advances in Chromatin Immunoprecipitation Sequencing and Its Application in Horticultural Plants

近年来,基于染色质免疫共沉淀技术(ChIP)与第二代测序技术结合的染色质免疫共沉淀测序技术(ChIP-seq)已被广泛用于研究DNA与蛋白质的相互作用,对解析园艺植物基因的表达与调控、靶基因筛选与定位、调控网络构建、染色质开放程度确认等方面具有较大的优势。本文综述了ChIP-seq技术的发展简史及其在园艺植物转录因子和组蛋白修饰中所取得的进展,以期为园艺植物DNA—蛋白互作研究提供参考。

In recent years,chromatin immunoprecipitation sequencing technology(ChIP-seq)based on the combination of chromatin immunoprecipitation technology(ChIP)and second-generation sequencing technology has been widely used to study the interaction between DNA and proteins.It has great advantages in analyzing the expression and regulation of horticultural plant genes,screening and mapping of target genes,construction of regulatory networks,and confirmation of chromatin openness.This paper reviews the development history of ChIP-seq technology and its progress in the modification of transcription factors and histones in horticultural plants,with the aim of providing theoretical reference for the study of DNA-protein interactions in horticultural plants.