槟榔愈伤中槟榔碱的提取方法

Analysis of extraction method of arecoline from areca callus

为了建立一种简便、快速并且样品用量少的测定槟榔愈伤中槟榔碱含量的方法,以槟榔(Areca catechu)愈伤为研究对象,采用超声波提取方法,选取料液比、提取时间、提取温度、提取次数4个因素,在单因素实验的基础上,进行L9(34)正交实验,根据单因素实验和正交实验的结果进行验证实验,研究了槟榔愈伤中槟榔碱的提取体系。结果表明:料液比1∶9、提取时间30 min、提取温度75℃、提取次数4次是最佳的槟榔碱提取体系。

In order to establish a simple,rapid and less sample method for the determination of arecoline in Areca catechu callus,an ultrasonic extraction method was used to extract arecoline from A.catechu calli,and four factors including solid-liquid ratio,extraction time,extraction temperature and extraction times were selected.On the basis of single factor experiment an orthogonal L9(34)experiment was performed to optimize the extraction system for arecoline from A.catechu calli.The results showed that the optimum extraction system for arecoline included the solid-liquid ratio of 1∶9,extraction time of 30 min,extraction temperature of 75℃and 4 extraction times.