香蕉枯萎病菌候选效应蛋白FoSSP20的鉴定和功能初探

Identification and functional analysis of candidate effector FoSSP20 in Fusarium oxysporum f.sp.cubense

为了鉴定课题组前期在Foc4基因组中筛选的候选效应蛋白的毒力功能,以从中选取的FoSSP20为研究对象,通过生物信息学分析其结构,并通过信号肽分泌实验、亚细胞定位实验、烟草瞬时表达实验和qRT-PCR初步分析其功能,结果显示,利用酵母分泌实验验证FoSSP20的信号肽的分泌功能,亚细胞定位分析表明分泌蛋白定位于烟草(Nicotiana benthamiana)细胞的细胞膜和细胞核中。qRT-PCR结果分析表明FoSSP20在香蕉枯萎病菌侵染前期显著上调表达。以上结果表明,FoSSP20是一个经典分泌蛋白,可能在病原菌侵染过程中发挥作用。

Fusarium wilt of banana caused by Fusarium oxysporum f.sp.cubense(Foc)is destructive to banana(Musa spp.)industry.Effector proteins play a key role in the interaction between Foc and banana.Previously researches screened the candidate effector FoSSP20 in Foc race 4(Foc4)which could suppress BAX-triggered programmed cell death(PCD).In this study,yeast secretion system was used to verify the secretory function of signal peptide of FoSSP20.Subcellular localization assay showed that the FoSSP20 was localized in the nucleus and plasma membranes in Nicotiana.benthamiana cells.qRT-PCR assay showed that the expression levels of FoSSP20 was significantly up-regulated at the early infection phase.All the results showed that effector FoSSP20 is a classic secretory protein which may function well in the infection process of pathogens.