绞股蓝提取物用于护肤品的功效初探

A Preliminary Study on the Efficacy of Gynostemma Extract for Skin Care Applications

在温度为90℃,m(绞股蓝粉末)∶V(80%乙醇)=1 g∶20 mL,回流2 h条件下,绞股蓝提取率为13.75%,黄酮质量分数为1.34%。绞股蓝提取物在质量浓度达0.04 mg/mL时,清除羟基自由基的能力与VC相近,可达85%;在质量浓度达0.018 mg/mL时,对DPPH自由基清除率达89.64%。绞股蓝提取物对酪氨酸酶活性的抑制率达最高时,质量浓度为600μg/mL,抑制率为57.58%。绞股蓝提取物同1%丙二醇相比,有一定的保湿功效。绞股蓝提取物能有效抑制大肠杆菌、金黄色葡萄球菌,绞股蓝提取物在质量浓度为2.43 mg/mL时有较佳的抗炎效果。对绞股蓝提取物抗氧化、美白保湿、抑菌等方面进行了完整评价,为其开发利用提供了参考。

Under the extraction conditions of 90℃,gibberellic acid powder∶80%ethanol=1 g∶20 mL,and reflux time of 2 h,the extraction rate of gibberellic acid was 13.75%,and its flavonoid content was measured to be 1.34%.The hydroxyl radical scavenging ability of gibberellic acid extract was similar to that of VC up to 85%at a concentration of 0.04 mg/mL,and the scavenging rate of DPPH radical reached 89.64%at a concentration of 0.018 mg/mL;the inhibition rate of gibberellic acid extract on the activity of tyrosinase reached the highest when the concentration of 600μg/mL,and the inhibition rate was 57.58%,and the extract of gibberellic acid compared with 1%propylene glycol,gibberellic acid extract has certain moisturizing effect;gibberellic acid extract can effectively inhibit Escherichia coli and Staphylococcus aureus,and gibberellic acid extract has better anti-inflammatory effect at a concentration of 2.43 mg/mL.This study provides a complete evaluation of the antioxidant,whitening and moisturizing,and antibacterial aspects of gynostemma extract,which provides a reference for its development and utilization.