云南烤烟KRK26特征香气分析

Analysis of Characteristic Aroma of Flue-cured Tobacco KRK26 in Yunnan Province

【目的】探究烤烟品种KRK26香韵风格,分析其重要致香成分及内在关系,为开发其工业可用性提供参考依据。【方法】通过感官辅助凝胶渗透色谱(gel permeation chromatography,GPC)分离筛选出关键香韵特征组分,采用气相色谱—质谱联用仪分析确定特征组分的重要致香成分,并结合相对香气活力值进行关键香气成分分析。【结果】感官辅助GPC分离得到5个(F2~F6)具有明显香韵特征的组分,其中,F2~F3清香香韵明显,F5~F6则呈焦甜香香韵。F2组分香韵特征以清甜香为主,辅以豆香,关键香气成分为金合欢醇和对甲基苯乙酮;F3组分带有强烈的清香,伴有花香和干草香韵,关键香气成分为金合欢醇和α-亚麻酸;F4组分以浓烈的花香和果香为主,底部有浓烈干草香香韵,关键香气成分为(+)-柠檬烯、柠檬醛、茄酮和香叶醇等;F5组分以苯乙酸带有的蜜甜香为主,辅以轻微焦甜香香韵;F6则具有强烈的焦甜香香韵,关键香气成分为麦芽酚和5-羟甲基糠醛等。【结论】KRK26香气风格以清香和焦甜香香韵为主,辅以花香和果香等香韵特征。

[Purpose]To explore the flavor style of flue-cured tobacco variety KRK26,analyzing the important aroma components and internal relations,and to provide reference for the industrial availability of KRK26.[Methods]Sensory as the guide,the key characteristic aroma components were separated and screened by gel permeation chromatography(GPC),and the aroma components of the characteristic components were determined by GC-MS method,and the key aroma components were analyzed in combination with the relative odor activity value(ROAV).[Results]Five com-ponents(F2-F6)were obtained by sensory assisted GPC.Among them,F2-F3 showed delicate fra-grance,while F5-F6 showed caramel aroma.The F2 component showed sweet fragrance with bean scent,the key aroma components were(2E,6E)-farnesol and 4′-methylacetophenone;the F3 compon-ent showed strong fragrance,floral and hay,the key aroma components were(2E,6E)-farnesol and li-nolenic acid;F4 fragrance with strong floral fragrance,fruit fragrance,the bottom of the strong hay scent,the key aroma components were(+)-limonene,citral,6,8-nonadien-2-one,8-methyl-5-(1-methylethyl)-,(5S,6E)-,2,7-dimethyl-2,6-octadiene and so on;the F5 component showed mainly honey sweet from phenylacetic acid;with a slight caramel aroma,while maltol,5-hydroxymethylfur-fural and other aroma components were found in F6 with a strong caramel aroma.[Conclusion]The aroma style of KRK26 is mainly fragrance and caramel aroma,supplemented by floral and fruity fra-grance.