米糠油中呈色物质的分离和鉴定

Isolation and Identification of Chromogenic Substances in Rice Bran Oil

米糠油的色泽是影响其品质的因素之一。该研究对米糠油中呈色物质的提取方法进行优化,并利用高效液相色谱串联质谱法对呈色物质进行分离和鉴定。以甲醇∶水(8∶2,体积比)为萃取剂,用液液萃取法提取米糠油中的强极性呈色物质,然后将样品皂化后利用正己烷提取弱极性呈色物质。强极性和弱极性提取液经质谱分别鉴定出9类物质,共37种,其中强极性提取液中二氢异黄酮类含量最多,弱极性提取液中二氢黄酮醇类为主要化合物。

Color is a factor affecting the quality of of rice bran oil.In this paper,the extraction method of chro-mogenic substances in rice bran oil was optimized,and the chromogenic substances were separated and identi-fied by high-performance liquid chromatography-tandem mass spectrometry.The strongly polar chromogenic substances in rice bran oil were extracted by liquid-liquid extraction with methanol∶water(8∶2,volume ratio)as extractant.Then,the samples were saponified and the weakly polar chromogenic substances were extracted using n-hexane.Nine categories of substances were identified from the strongly polar and weakly polar extracts separately,37 substances in total.Dihydroisoflavones was the highest in the strongly polar extract,and dihy-droflavonols were the main compounds in the weakly polar extract.