补骨脂盐炙前后HPLC指纹图谱的比较

Comparison of HPLC fingerprints of Psoralea corylifolia L.before and after salt roasting

建立补骨脂(Psoralea corylifolia L.)盐炙前后的高效液相(HPLC)指纹图谱,并采用化学计量学方法评价生品补骨脂、盐补骨脂的差异。采用HPLC法建立指纹图谱,色谱柱为Waters Symmetry C18柱(4.6 mm×150 mm,5μm),流速为1.0 mL/min,柱温为30℃,进样量为10μL,检测波长为290 nm。采用镜像对比、偏最小二乘判别(PLS-DA)、相似性评价分析方法进行数据分析。成功建立了生品补骨脂、盐补骨脂的HPLC指纹图谱,共确定14个共有峰,并指认了补骨脂素、异补骨脂素2种成分。生品补骨脂相似度良好,盐炙后的补骨脂相似度发生变化。镜像分析结果表明,盐炙后的补骨脂HPLC指纹图谱与生品补骨脂存在差异。与生品补骨脂相比,盐补骨脂的1~14号峰均有不同程度的增加。PLS-DA分析结果表明,生品补骨脂与盐补骨脂在化学成分上存在差异。建立的指纹图谱方法重复性、稳定性良好,可为补骨脂盐炙前后的鉴别及质量分析提供参考。

A high-performance liquid chromatography(HPLC)fingerprint of Psoralea corylifolia L.before and after salt roasting was established,and the differences between raw Psoralea corylifolia L.and salted Psoralea corylifolia L.were evaluated by using chemo⁃metric methods.HPLC method was used to establish the fingerprint,and the chromatographic column was Waters Symmetry C18 col⁃umn(4.6 mm×150 mm,5μm).The flow rate was 1.0 mL/min,the column temperature was 30℃,the injection volume was 10μL,and the detection wavelength was 290 nm.Data analysis was conducted using mirror comparison,partial least squares discriminant analysis(PLS-DA),and similarity evaluation analysis methods.HPLC fingerprints of raw Psoralea corylifolia L.and salted Psoralea corylifolia L.were successfully established,identified a total of 14 common peaks were,and determined two components,psoralen and isopsoralen,were identified.Raw Psoralea corylifolia L.had good similarity,and the similarity of Psoralea corylifolia L.after salt roasting changed.The mirror analysis results indicated that there were differences in the HPLC fingerprint of Psoralea corylifolia L.af⁃ter salt roasting compared to raw Psoralea corylifolia L..Compared with raw Psoralea corylifolia L.,the peaks 1~14 of salted Psoralea corylifolia L.had increased to varying degrees.The PLS-DA analysis results indicated that there were differences in chemical composi⁃tion between raw Psoralea corylifolia L.and salted Psoralea corylifolia L.The established fingerprint method had good repeatability and stability,and could provide reference for the identification and quality analysis of Psoralea corylifolia L.before and after salt roasting.