不同核数的灵芝菌丝体转录组比较分析

Comparative analysis of transcriptome of Ganoderma lucidum mycelium with different nuclear numbers

以野生灵芝[Ganoderma lucidum(Leyss. ex Fr.)Karst.](D)、自然单核灵芝(Mo)、双核灵芝(Mu)的菌丝体为材料,利用高通量测序技术筛选差异表达基因,并进行GO分析和KEGG富集分析。通过差异基因分析,筛选出568个差异表达基因。GO分析结果表明,野生灵芝(D)与自然单核灵芝(Mo)菌丝体的差异表达基因主要富集在转录调控及DNA模板化、tRNA氨酰化、组蛋白甲基化等过程;野生灵芝(D)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、L-丝氨酸代谢、硫化合物代谢等过程;自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体的差异表达基因主要富集在染色质沉默、DNA模板转录、谷氨酸分解等过程。KEGG富集结果表明,自然单核灵芝(Mo)与双核灵芝(Mu)菌丝体在代谢通路上有明显的差异,差异基因主要被注释到过氧化物酶、维生素代谢等通路。野生灵芝(D)与自然单核灵芝(Mo)菌丝体、野生灵芝(D)与双核灵芝(Mu)菌丝体的差异基因并没有KEGG富集的结果,说明其代谢通路没有明显差异。筛选出3个品系中表达量差异均显著的基因,共12个,并对基因的主要功能进行预测。12个基因中有5个基因可以匹配到GO库。GO注释结果表明,这5个基因主要被注释到氧化还原酶活性、金属离子结合、膜的调控与膜组成等方面。

Using the mycelium of wild Ganoderma lucidum[Ganoderma lucidum(Leyss.ex Fr.)Karst.](D),natural mononuclear Ganoderma lucidum(Mo),and binuclear Ganoderma lucidum(Mu)as materials,high-throughput sequencing technology was used to screen differentially expressed genes,and GO analysis and KEGG enrichment analysis were conducted.Through differential gene analysis,568 differentially expressed genes were screened.The GO analysis results showed that the differentially expressed genes be⁃tween wild Ganoderma lucidum(D)and natural mononuclear Ganoderma lucidum(Mo)hyphae were mainly enriched in transcription⁃al regulation,DNA templating,tRNA aminoacylation,and histone methylation processes;the differentially expressed genes in the my⁃celium of wild Ganoderma lucidum(D)and binuclear Ganoderma lucidum(Mu)were mainly enriched in processes such as chromatin silencing,L-serine metabolism,and sulfur compound metabolism;the differentially expressed genes in the mycelium of natural mono⁃nuclear Ganoderma lucidum(Mo)and binuclear Ganoderma lucidum(Mu)were mainly enriched in processes such as chromatin si⁃lencing,DNA template transcription,and glutamate decomposition.The KEGG enrichment results indicated that there were signifi⁃cant differences in the metabolic pathways between natural mononuclear Ganoderma lucidum(Mo)and binuclear Ganoderma lucidum(Mu)mycelium,with differential genes mainly annotated into pathways such as peroxidase and vitamin metabolism.The differential genes between wild Ganoderma lucidum(D)and natural mononuclear Ganoderma lucidum(Mo)mycelium,as well as between wild Ganoderma lucidum(D)and binuclear Ganoderma lucidum(Mu)mycelium,did not show KEGG enrichment,indicating no signifi⁃cant differences in their metabolic pathways.A total of 12 genes with significant differences in expression levels were selected from 3 strains,and the main functions of the genes were predicted.Out of 12 genes,5 genes could be matched to the GO library.The GO anno⁃tation results indicated that these 5 genes were mainly annotated in aspects such as oxidoreductase activity,metal ion binding,mem⁃brane regulation,and membrane composition.