基于RPA的病原体快速诊断策略

RPA-based Rapid Diagnostic Strategies for Pathogens

重组酶聚合酶扩增(recombinase enzyme polymerase amplification,RPA)检测技术是2006年出现的一种等温核酸扩增检测技术。RPA所需引物设计简单,反应时间短,在37~42℃下仅需15~20 min即可达到病原体检测水平,搭配便捷装置即可实现核酸检测,近些年得到快速发展。重点介绍了RPA反应产物检测的相关策略,并对RPA在致病菌、病毒以及寄生虫上的快速诊断应用研究进行述评。

Recombinase polymerase amplification(RPA)is an isothermal nucleic acid amplification technique that emerged in 2006.The primer design for RPA is simple,and the reaction time is short.At temperatures ranging from 37 to 42℃,it takes only 15~20 min to reach the pathogen detection level.When coupled with convenient devices,it can build a nucleic acid detection platform.In recent years,RPA has undergone rapid development.This article focuses on the strategies for detecting RPA reaction products and provides a review of the rapid diagnostic application of RPA in pathogenic bacteria,viruses,and parasites.