受体相互作用蛋白激酶3特异性抑制剂GSK872联合伊木萨克片对糖尿病性勃起功能障碍大鼠勃起功能的影响

Effect of receptor interaction protein kinase-3 specific inhibitor GSK872 combined with Yimusake tablets on erectile function of diabetic mellitus erectile dysfunction rats

目的探究受体相互作用蛋白激酶3(RIP3)特异性抑制剂GSK872联合伊木萨克片对糖尿病性勃起功能障碍(DMED)大鼠勃起功能的影响。方法150只雄性SD大鼠随机选取20只为正常对照组(N组),其余130只通过连续2 d注射链脲佐菌素(45 mg/kg)建立DMED大鼠模型。造模8周后筛选出DMED大鼠115只,随机选取80只分为DMED模型组(DMED组)、GSK872干预组(RIP3i组)、伊木萨克片干预组(Y组)及GSK872与伊木萨克片联合干预组(RIP3i+Y组),每组20只。干预14 d后,通过随机血糖检测、阿扑吗啡(APO)实验和性行为学实验综合评价干预效果。结果与N组比较,DMED组大鼠随机血糖升高,阴茎勃起能力和性功能降低(P<0.05);与DMED组比较,RIP3i组、Y组及RIP3i+Y组大鼠随机血糖差异无统计学意义(P>0.05),但阴茎勃起能力和性功能升高(P<0.05),其中RIP3i+Y组升高更显著(P<0.05)。结论RIP3特异性抑制剂GSK872和伊木萨克片干预均可改善DMED大鼠勃起功能,而二者联合的干预效果优于单独干预。

Objective To investigate the effects of receptor interaction protein kinase-3(RIP3)specific inhibitor GSK872 combined with Yimusake tablets on erectile function of diabetic mellitus erectile dysfunction(DMED)rats.Methods Among 150 male SD rats,20 were randomly selected as normal control group(N group),and the remaining 130 rats were injected with streptozotocin(45 mg/kg)for two consecutive days to induce DMED rat models.After 8 weeks of modeling,115 DMED rats were screened and 80 rats were randomly selected and divided into DMED model group(DMED group),GSK872 intervention group(RIP3i group),Yimusake tablets intervention group(Y group)and GSK872 combined with Yimusake tablets intervention group(RIP3i+Y group),with 20 rats in each group.After 14 days intervention,the effect of intervention was comprehensively evaluated by random blood glucose test,apomorphine(APO)test and sexual behavioural test.Results Compared with N group,the rats in DMED group showed increased random blood glucose,and decreased erectile function and sexual function(P<0.05).Compared with DMED group,there were no significant differences in random blood glucose of rats in RIP3i group,Y group and RIP3i+Y group(P>0.05),but the erectile function and sexual function increased(P<0.05),with the increase in RIP3i+Y group being more significant(P<0.05).Conclusions RIP3 specific inhibitor GSK872 and Yimusake tablets can both improve erectile function in DMED rats,and the effect of combined intervention is superior than that of single intervention.