基于形态标记和ISSR分子标记的长瓣铁线莲遗传多样性分析

Genetic Diversity Analysis of Clematis macropetala Based on Morphological Markers and ISSR Molecular Makers

为快速区分鉴定市场上繁多的长瓣铁线莲(Clematis macropetala)品种,了解长瓣铁线莲间的遗传多样性水平及亲缘关系,本试验利用形态标记结合ISSR分子标记对25个长瓣铁线莲品种和1个野生种进行遗传多样性研究。结果表明:14个数量形状的变异系数在0.24%~2.32%之间;15个假质量性状的信息多样性指数H在0~2.29%之间,遗传多样性指数D在0~0.94%之间;在欧式距离为15时,可将29个表型性状划分为七类,在欧式距离为15时,可将26个长瓣铁线莲划分为五类。12条引物共扩增出144个条带,多态性条带占比为100%。利用引物UBC815、UBC824和UBC836构建26个长瓣铁线莲的指纹图谱,可快速区分鉴定26个长瓣铁线莲。UPGMA聚类结果显示,在遗传相似系数为0.68时,可将26个长瓣铁线莲划分为八大类。形态标记结合ISSR分子标记可有效鉴别长瓣铁线莲种质资源,以期为长瓣铁线莲种质资源收集保存、创制新品种等提供理论基础。

This experiment aimed to quickly distinguish and identify the varieties of Clematis macropetala on the market,and to understand diversity and relationships among 26 Clematis macropetala samples.The experiment used morphological markers combined with ISSR molecular markers to study genetic diversity and phylogenetic relationships among 26 Clematis macropetala.The results showed that the variation coefficient of 14 quantitative shapes ranged from 0.24%to 2.32%,with an average coefficient of variation of 0.29%for leaf indicators and 0.64%for flower indicators.The information diversity index H of 15 quality traits ranged from 0 to 2.29%,and the genetic diversity index D ranged from 0 to 0.94%.At a European distance of 15,29 phenotypic traits were divided into 7 categories,and at a European distance of 15,26 samples of Clematis macropetala were divided into 5 categories.The total of 144 bands were amplified using 12 primers,with a polymorphism rate of 100%.Using primers UBC815,UBC824,and UBC836 to construct fingerprint maps of 26 Clematis macropetala,26 Clematis macropetala were quickly distinguished and identified.The UPGMA clustering analysis results showed that when the genetic similarity coefficient was 0.68,26 Clematis macropetala could be divided into 8 categories.Phenotypic traits combined with ISSR molecular markers could effectively identify the germplasm resources of Clematis macropetala.These research results provided a theoretical basis for the collection and preservation of Clematis macropetala germplasm resources,as well as the creation of new varieties.