实时荧光聚合酶链式反应检验用于乙型肝炎病毒DNA检测的价值分析

Value Analysis of Real-time Fluorescence Polymerase Chain Reaction Test in Hepatitis B Virus DNA Detection

目的:探讨实时荧光聚合酶链式反应(PCR)检验用于乙型肝炎病毒(HBV)DNA检测中的价值。方法:选取2020年1月—2023年1月清镇市第一人民医院收治的乙型肝炎患者112例为研究对象,均经标准abbot药盒检测确诊,包括HBsAg(+)、HBcAb(+)、HBeAg(+)(临床习惯称为“大三阳”)62例、HBsAg(+)、HBcAb(+)、HBeAb(+)(临床习惯称为“小三阳”)50例。患者均接受酶联免疫吸附法检验与实时荧光PCR检验HBV-DNA。将标准abbot药盒检测结果作为金标准,评价酶联免疫吸附法检验与实时荧光PCR检验的诊断效能。结果:临床最终诊断结果显示“大三阳”62例(阳性)、“小三阳”50例(阴性),酶联免疫吸附法检出阳性50例、阴性62例,实时荧光PCR检验检出阳性58例、阴性54例;实时荧光PCR检验诊断准确度、特异度、灵敏度高于酶联免疫吸附法检验,差异有统计学意义(P<0.05)。结论:与酶联免疫吸附法检验比较,HBV-DNA检测中采取实时荧光PCR检验,可提高对“大三阳”与“小三阳”的诊断效能。

Objective:To explore the value of real-time fluorescence polymerase chain reaction(PCR)test in the DNA detection of hepatitis B virus(HBV).Methods:A total of 112 patients with HBV who were admitted to the First People's Hospital of Qingzhen from January 2020 to January 2023 were selected as the study subjects.All the patients were confirmed by standard abbot kit testing,including 62 cases of HBsAg(+)、HBcAb(+)、HBeAg(+)(In chinical practice is called"positive major three items")and 50 cases of HBsAg(+)、HBcAb(+)、HBeAb(+)(In chinical practice is called"positive minor three items").All the patients underwent enzyme-linked immunosorbent assay test and real-time fluorescence PCR test for hepatitis B virus DNA.The results of standard abbot kit testing were used as the golden standard to evaluate the diagnostic efficiency of enzyme-linked immunosorbent assay test and real-time fluorescence PCR test.Results:The final clinical diagnosis results showed that there were 62 cases of positive major three items(positive)and 50 cases of positive minor three items(negative);50 cases were positive and 62 cases were negative by enzyme-linked immunosorbent assay,and 58 cases were positive and 54 cases were negative by realtime fluorescence PCR test;the diagnostic accuracy,specificity and sensitivity of real-time fluorescence PCR test were higher than those of enzyme-linked immunosorbent assay,and the difference was statistically significant(P<0.05).Conclusion:Realtime fluorescence PCR test is adopted in the detection of hepatitis B virus DNA,which can improve the positive rate of the detection of positive major three items and positive minor three items,and the diagnostic efficiency is higher.