化湿通痹方对勃起功能障碍大鼠作用及机制研究

Study on the effect and mechanism of Huashi Tongbi formula on erectile dysfunction rats

目的 揭示化湿通痹方(HTF)对大鼠勃起功能障碍(ED)的作用及疗效机制。方法 SD大鼠分为空白对照组、假手术组、模型对照组、西地那非组、HTF低中高剂量组,每组7只。西地那非组予西地那非水溶液灌胃,HTF组分别予HTF水溶液低、中、高剂量灌胃,其他三组予生理盐水灌胃,干预6周,测量ICP、MAP,计算ICP/MAP,检测血清TC、HDL-C、LDL-C、CRP、IL-6及NO。结果 与空白对照组比较,模型对照组阴茎勃起次数减少、ICP/MAP降低(P<0.05),TC、LDL-C升高、HDL-C降低(P<0.05),CRP、IL-6升高、NO降低(P<0.05)。与模型对照组比较,HTF中剂量组阴茎勃起次数增加(P<0.05),HTF各剂量组LDL-C下降、HDL-C升高(P<0.05),CRP和IL-6降低,NO升高(P<0.05)。结论 HTF改善大鼠血脂异常,抑制炎性反应,提高血清NO治疗大鼠ED。

Objective To explore the effect of Huashi Tongbi formula(HTF)in treating erectile dysfunction(ED)in rats and its therapeutic mechanism.Methods SD rats were divided into control group,sham surgery group,model control group,sildenafil group,and HTF low,medium,and high dose group,with 7 rats in each group.The sildenafil group was gavaged with sildenafil aqueous solution,the HTF group was gavaged with low,medium,and high doses of HTF aqueous solution,and the other three groups were gavaged with physiological saline for 6 weeks of intervention.ICP and MAP were measured,ICP/MAP was calculated,and serum TC,HDL⁃C,LDL⁃C,CRP,IL⁃6,and NO.Results Compared with the control group,the model control group showed a decrease in penile erection frequency,a decrease in ICP/MAP(P<0.05),an increase in TC and LDL⁃C,a decrease in HDL⁃C(P<0.05),an increase in CRP,IL⁃6,and a decrease in NO(P<0.05).Compared with the model control group,the moderate dose group of HTF increased the number of penile erections(P<0.05),while the various dose groups of HTF decreased LDL⁃C and increased HDL⁃C(P<0.05),decreased CRP and IL⁃6,and increased NO(P<0.05).Conclusion HTF improves dyslipidemia,inhibits inflammatory reactions,and increases serum NO levels for the treatment of ED in rats.