不同温度下致倦库蚊卵孵化观察

Observation on hatchability of Culex quinquefasciatus eggs at different temperatures

目的了解致倦库蚊卵在不同温度下孵化变化情况,为室内致倦库蚊饲养和室外致倦库蚊幼虫监测提供参考数据。方法将48只致倦库蚊卵筏随机放入加满水的细胞板的水孔中,分别放置于18℃、22℃、26℃、28℃、30℃,每天计数孵化幼虫;用SPSS 26.0软件分析卵粒数、孵化率等数据。结果计数的60只致倦库蚊卵筏平均含有142粒卵,最少为60粒,最多为234粒;致倦库蚊卵孵化率最高为95.93%,最低为69.44%,孵化率差异无统计学意义(χ^(2)=5.892,P=0.552);91.59%的致倦库蚊卵在1 d内孵化;致倦库蚊卵的孵化率不受试验温度、湿度变化影响(r=0.037,P=0.823;r=0.081,P=0.619);孵化时间随着温度升高而缩短,与温度呈负相关性(r=-0.460,P=0.003)。结论温度对致倦库蚊卵孵化率影响不明显,18℃时大部分卵延长孵化时间。

Objective To study the hatchability of Culex quinquefasciatus eggs at different temperatures,provide reference for breeding of Cx.quinquefasciatus indoors and surveillance of larva of Cx.quinquefasciatus outdoors.Methods A total of 48 Cx.quinquefasciatus egg rafts were randomly put into wells of cell culture plate with full of water,which were placed at 18℃,22℃,26℃,28℃,30℃.The larvae were sucked out and counted every day.Software SPSS 26 was applied to perform analysis of eggs and hatching rates.Results 60 Cx.quinquefasciatus egg rafts included 142 eggs on average with a minimum of 60 and a maximum of 234.The highest hatchability of Cx.quinquefasciatus eggs was the highest(95.93%)and the lowest was 69.44%,there was no significant difference in hatchability(χ^(2)=5.892,P=0.552).91.59%eggs hatched within 1 d.Hatching rate was not significantly correlated with temperature(r=0.037,P=0.823;r=0.081,P=0.619).Hatching time reduced when temperature rose,and they were in a negative correlation(r=-0.460,P=0.003).Conclusion Temperature had no significant effect on the hatchability of Cx.quinquefasciatus eggs,and most eggs would prolong hatching time at 18°C.