硫自养反硝化系统处理化工园区尾水特征分析

Characteristic analysis of tail water treatment in chemical industry parks by sulfur autotrophic denitrification system

采用硫自养反硝化装置对某农药化工园区水处理系统末端排水进行脱氮处理,系统成功启动且运行平稳后,在室温约16℃,HRT为22 h情况下,分别运行硝酸盐模拟废水和实际废水。试验结果表明,以NO3--N模拟废水运行25 d,NO_(3)^(-)-N平均去除率为87%,出水平均浓度为21 mg/L;以实际尾水运行20 d,TN平均去除率为88%,出水平均浓度为17mg/L,反硝化系统脱氮效果良好且运行稳定。对反应器内不同阶段的微生物进行高通量测序分析,在门水平上的结果表明Proteobacteria为占比最大的菌门,Campilobacterota由模拟废水过渡到实际废水运行阶段,丰富度显著增加。在属水平上Sulfurimonas和Thiobacillus为系统内存在的典型菌属,二者具有硫自养反硝化功能,反应后丰度均有较大提升。其中Thiobacillus表现在硫自养反硝化功能上表现出优势,在无机硫的氧化和硝酸盐的还原中发挥了重要作用。

A sulfur autotrophic denitrification plant was used to denitrify the end drainage of a pesticide chemical park water treatment system.After the system was successfully started and operated smoothly,the nitrate simulated wastewater and the actual wastewater were run at room temperature of about 16℃and HRT of 22 h.The test results showed that the average removal rate of NO_(3)^(-)-N was 87% and the average concentration of effluent was 21 mg/L after running for 25 d in the simulated wastewater,and the average concentration of the effluent was 17 mg/L,indicating that the denitrification system had good denitrification effect and stable operation.High-throughput sequencing analysis of microorganisms at different stages in the reactor was carried out.The results at the phylum level showed that Proteobacteria was the largest bacterial phylum,and Campilobacterota transitioned from the simulated wastewater to the actual wastewater operation stage,and the abundance increased significantly.At the genus level,Sulfurimonas and Thiobacillus were typical bacterial genera existing in the system.Both of them had the function of sulfur autotrophic denitrification,and their abundance was greatly improved after the reaction.Thiobacillus showed superiority in sulfur autotrophic denitrification and played an important role in inorganic sulfur oxidation and nitrate reduction.