肾虚三证大鼠模型子宫血管构筑的比较研究

Comparative Study on Uterine Vascular Architecture of Kidney Deficiency Three Syndrome Rat Models

目的探讨肾虚三证对雌鼠子宫血管构筑的影响。方法将雌性SD大鼠随机分为对照组、肾精亏虚组、肾阴虚组和肾阳虚组,每组12只。分别予反复超促排卵5次、甲状腺片联合利血平混悬液灌胃连续21日和4℃羟基脲混悬液灌胃连续21日构建肾精亏虚、肾阴虚和肾阳虚模型。造模成功后观察15日,于动情后期处死大鼠,各组随机选取8只大鼠取血及子宫,ELISA法测定血清促卵泡激素(follicle-stimulating hormone,FSH)、抗谬勒管激素(anti-meulerian hormone,AMH)、雌二醇(estrogen,E 2)、孕酮(progesterone,P)水平,苏木精-伊红法(hematoxylin-eosin staining,HE)染色并测量子宫内膜厚度,计数腺体数。剩余大鼠采用硫酸钡溶液进行血管灌注,微计算机断层扫描(microcomputed tomography,Micro-CT)观察并量化子宫血管构筑。结果与对照组比较,三模型组大鼠血清FSH显著升高,E 2、P、AMH和血管体积占比均下降(P<0.05);肾精亏虚组大鼠子宫内膜厚度,腺体数,子宫二、三、四级血管数减少(P<0.05);肾阴虚组大鼠子宫出血,一、二级血管平均直径和二、三、四级血管数减少(P<0.05);肾阳虚组大鼠子宫内膜厚度、腺体数、子宫各级血管平均直径减少(P<0.05)。结论肾虚三证能够造成雌鼠子宫内膜形态改变、容受性下降,子宫血管构筑的变化可能是原因之一。

Objective To investigate the effect of three syndromes of deficiency of kidney essence,deficiency of kidney Yin and deficiency of kidney Yang on uterine angioarchitecture in female rats.Methods Female SD rats were randomly divided into four groups,control group,kidney essence deficiency group,kidney Yin deficiency group and kidney Yang deficiency group,12 rats in each group.The three kidney deficiency models were established by repeated superovalation for 5 times,intragastric administration of thyroid tablets combined with serpine suspension for 21 days,and intragastric administration of hydroxyurea suspension at 4℃for 21 days.After 15 days observation,the rats were sacrificed at the late stage of estrus.Blood and uterus were collected from 8 rats randomly selected from each group.Serum follicle-stimulating hormone(FSH),anti-meulerian hormone(AMH),estrogen(E 2),progesterone(P)levels were measured by ELISA.The endometrial thickness was measured and the number of glands was counted by HE staining.The remaining rats were perfused with barium sulfate solution.Uterine angioarchitecture was observed and quantified by Micro-CT.Results Compared with the control group,the content of FSH in serum of the three model groups was significantly increased(P<0.01).The content of serum E 2,P and AMH was significantly decreased,and the vascular volume proportion of the three models was decreased as well(P<0.05).In kidney essence deficiency group,the endometrial thickness,the number of endometrial gland,and the number of second,third and fourth blood grade vessels in uterus was significantly decreased(P<0.05).In kidney Yin deficiency group,abnormal uterine bleeding was observed,the mean diameter of primary and secondary grade blood vessels decreased,and the number of secondary,tertiary and quaternary grade blood vessels decreased significantly(P<0.05).In kidney Yang deficiency group,the endometrial thickness,the number of endometrial gland,and the average diameter of uterine blood vessels at all levels decreased(P<0.05).Conclusion The morphological changes and decreased receptivity of endometrium in rats were observed in three kidney deficiency models.The changes of uterine angioarchitecture may be one of the reasons.