紫杉醇联合卡铂化疗对卵巢癌VEGF及肿瘤标志物表达的影响

Effects of paclitaxel combined with carboplatin on expression of VEGF and tumor markers in ovarian cancer

目的探讨紫杉醇与卡铂联合化疗对卵巢癌(OC)患者血细胞相关指标、血管内皮生长因子(VEGF)及肿瘤标志物的影响。方法OC患者联合化疗(CC)组和卵巢良性肿瘤对照(control)组,各组50例。HE染色观察OC卵巢细胞病理改变,免疫组化染色(IHC)观察VEGF表达;流式细胞术(FC)检测血细胞数量;电化学发光法(ECL)检血清糖类抗原125(CA125)和人附睾蛋白4(HE4)水平。结果与对照组相比,OC卵巢织织结构紊乱,细胞异型明显;VEGF表达明显增强,血小板(PLT)计数、CA125和HE4水平显著升高(P<0.01)。但化疗后CC组卵巢组织可见坏死细胞、VEGF表达减弱、PLT计数、CA125和HE4水平较对照组显著降低(P<0.01)。结论联合化疗对OC患者VEGF表达以及CA125、HE4水平具有调节作用。

Objective To explore the effect of paclitaxel with carboplatin combined chemotherapy on hemocyte related indexes,vascular endothelial growth factor(VEGF)and tumor markers in ovarian cancer(OC)patients.Methods OC patients combined chemotherapy(CC)group and ovarian benign tumor control group with 50 cases in each.The pathological changes in the ovarian cells were observed by HE staining and the expression of VEGF was observed by immunohistochemical staining(IHC).Blood cell was counted by flow cytometry(FC).Serum carbohydrate antigen 125(CA125)and human epididymal protein 4(HE4)were measured by electrochemical luminescence(ECL).Results Compared with control group,the ovarian tissue structure was disordered and the cell atypia was obvious in OC.VEGF expression was significantly enhanced.Platelet count(PLT),CA125 and HE4 were significantly increased(P<0.01).However,necrotic cells were observed in ovarian tissue of CC group.VEGF expression was inhibited.PLT count and the level of CA125 and HE4 were significantly lower than those of control group after chemotherapy(P<0.01).Conclusions Combined chemotherapy may regulate the level of VEGF,CA125 and HE4 in OC patients.