两种小鼠尾静脉采血法进行血糖检测的比较分析

Comparison and Analysis of Blood Glucose Detection by Two Collection Methods of Tail Vein Blood Sampling in Mice

目的比较刺破尾静脉采血法与剪尾采血法检测的小鼠血糖值,从而评价两种采血方法的可行性,探讨一种稳定、有效及符合实验动物伦理学的小鼠末梢静脉全血血糖值的科学检测实验方法。方法选取健康C57BL/6J小鼠,分别采用刺破尾静脉采血法和剪尾采血法采集血样检测其空腹血糖值,比较两种采血方法采集的血样所测血糖值的差异,并分析其相关性。结果刺破尾静脉采血法检测血糖值为(7.58±0.92)mmol/L,与剪尾采血法的(7.61±1.02)mmol/L比较,差异无统计学意义(P>0.05),但是刺破尾静脉采血法检测血糖与剪尾采血法检测血糖值有正线性趋势,回归分析方程式为:y(剪尾采血法)=1.898+0.746x(刺破尾静脉采血法)。结论刺破尾静脉采血与剪尾采血检测出来的血糖值无差异,两种采血方法检测的血糖值有正线性趋势。

Objective To compare the blood glucose values of mice detected by punctured tail vein blood collection method and cut tail blood collection method,so as to evaluate the feasibility of the two blood collection methods,and to explore a stable,effective and scientific experimental method for detecting the blood glucose value of peripheral vein blood in mice.Methods The healthy C57BL/6J mice were selected,and blood samples were collected by puncture tail vein blood collection method and cut tail blood collection method to detect their fasting blood glucose values.The differences in blood glucose values measured by blood samples collected by the two blood collection methods were compared,and their correlation was analyzed.Results The blood glucose value was(7.58±0.92)mmol/L by puncture tail vein blood collection method,which was compared with(7.61±1.02)mmol/L by cutting the tail blood collection method,the difference was not statistically significant(P>0.05).Whereas there was a positive linear trend between the detection of FBG by two blood collection methods and the regression analysis equation was:y(cut tail blood collection method)=1.898+0.746x(puncture tail vein blood collection).Conclusion There is no difference in blood glucose values detected by punctured tail vein blood collection method and cut tail blood collection method.The blood glucose values detected by the two blood collection methods have a positive linear trend.