摘要
为探究紫薇(Lagerstroemia indica)分枝性状的形成机制,以紫薇标准型和低矮紧凑型株系为材料,以分枝相关的生长素转运基因LfiPIN1、LfiPIN6和响应因子LfiARF9为对象,利用VIGS沉默(紫薇)和过表达(烟草Nicotiana tabacum)技术对3个基因进行功能分析。结果表明,LfiPIN6和LfiARF9通过抑制侧芽的萌发而负调控分枝的数量;LfiPIN1和LfiPIN6通过调节新生侧枝的生长素含量正调控侧枝的长度,而LfiARF9并不影响侧枝长度。
To investigate the mechanism underlying branching in Lagerstroemia indica,both standard and dwarf compact L.indica strains were used as material,the function of three genes involved in auxin transport and response,namely LfiPIN1,LfiPIN6 and LfiARF9,were analyzed using virus-induced gene silencing(VIGS)in L.indica and overexpression techniques in Nicotiana tabacum.The effects of these genes on lateral bud initiation and lateral branch length in L.indica were examined.The results showed that LfiPIN6 and LfiARF9 negatively regulate the number of lateral branches in L.indica by inhibiting the sprouting of lateral buds.LfiPIN1 and LfiPIN6 positively regulate the length of lateral branches by regulating the auxin content in newly formed lateral branches.LfiARF9 was found to have no effect on lateral branch length.
作者
黄佳玉
刘洁茹
康佳音
池秀凤
申萍
蔡明
程堂仁
王佳
张启翔
潘会堂
HUANG Jiayu;LIU Jieru;KANG Jiayin;CHI Xiufeng;SHEN Ping;CAI Ming;CHENG Tangren;WANG Jia;ZHANG Qixiang;PAN Huitang(Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment,College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第12期2619-2632,共14页
Acta Horticulturae Sinica
基金
国家重点研发计划项目(2019YFD1001004)。
关键词
紫薇
分枝
生长素
功能验证
Lagerstroemia indica
branching
auxin
function verification
