1株海洋真菌Talaromyces sp.HK1-18次级代谢产物及其抗菌活性研究

Secondary metabolites and antibacterial activity of the marine-derived fungus,Talaromyces sp.HK1-18

目的研究1株分离于海南东寨港红树林自然保护区海桑(Sonneratia caseolaris)根际土壤的真菌Talaromyces sp.HK1-18的次级代谢产物及其抗菌活性。方法利用正、反相硅胶柱层析、凝胶柱层析和半制备高效液相色谱等色谱分离方法,对该菌的次级代谢产物进行分离纯化;利用核磁共振波谱以及查阅参考文献等方法,鉴定化合物的结构;采用最小抑菌浓度(MIC)法对化合物进行抗菌活性评价。结果从该真菌中分离获得8个化合物(1~8),结构分别鉴定为penicillide(1)、purpactin A(2)、vermistatin(3)、deoxyfunicone(4)、dankasterone A(5)、fortisterol(6)、过氧化麦角甾醇(7)、radiclonic acid(8)。结论化合物6为首次从Talaromyces属真菌中分离得到。化合物1和8对多药耐药金黄色葡萄球菌(S.aureus ATCC 43300)显示出较强的抑制活性,MIC值均为0.78μg/mL。化合物6对耐甲氧西林金黄色葡萄球菌(S.aureus ATCC 33591)显示出较强的抑制活性,MIC值为0.78μg/mL。

Objective To study the secondary metabolites and antibacterial activity of the marine-derived fungus,Talaromyces sp.HK1-18,isolated from the Sonneratia caseolaris rhizosphere soil collected from Dongzhaigang Mangrove Nature Reserve,Hainan Province.Methods The secondary metabolites were isolated by silica gel column chromatography(CC),reversed phase silica gel CC,Sephadex LH-20 gel CC and semipreparative high performance liquid chromatography.Their structures were identified by nuclear magnetic resonance spectroscopy(NMR)and compared with the reported spectral data.The antibacterial activities of these compounds were evaluated by minimal inhibit concentration(MIC)method.Results Eight compounds(1-8)were isolated from Talaromyces sp.HK1-18.Their structures were identified as penicillide(1),purpactin A(2),vermistatin(3),deoxyfunicone(4),dankasterone A(5),fortisterol(6),ergosterol peroxide(7),and radiclonic acid(8).Conclusion Compound 6 was isolated from the genus of Talaromyces for the first time.Compounds 1 and 8 showed strong antibacterial activity against multidrug resistant S.aureus ATCC 43300with the same MIC value of 0.78μg/mL.Compound 6 displayed strong inhibitory activity against methicillin-resistant S.aureus ATCC 33591 with the MIC value of 0.78μg/mL.