珍珠通络丸对脑缺血再灌注损伤大鼠的神经保护作用研究

Neuroprotective effects of Zhenzhu Tongluo Pills on the cerebral ischemia reperfusion injury in rats

目的探讨珍珠通络丸对脑缺血再灌注损伤大鼠的保护作用。方法采用随机数字表法将15只健康雄性SD大鼠分为假手术组、模型组和中药组,每组5只。除假手术组外,其余2组采用线栓法制备大脑中动脉缺血再灌注损伤模型。造模成功后,中药组灌胃0.5 g/(kg·d)珍珠通络丸原粉溶液;模型组和假手术组灌胃等体积生理盐水,1次/d,连续灌胃14 d。采用Bederson方法评价神经功能缺损症状,TTC染色确定梗死体积并计算梗死比,HE染色法观察神经细胞损伤情况,免疫组化法检测各组缺血病灶周围脑组织胶质纤维酸性蛋白(GFAP)、巢蛋白(Nestin)、神经元特异核蛋白(NeuN)表达。结果末次给药后30 min,中药组Bederson评分低于再灌注3 h(P<0.05);与模型组比较,中药组大鼠梗死比缩小(P<0.05);模型组大鼠缺血灶周围神经元细胞NeuN染色浅淡,Nestin和GFAP染色较深;中药组大鼠神经元细胞Nestin和GFAP染色浅淡,NeuN染色较深。结论珍珠通络丸可改善脑缺血大鼠神经功能缺损症状,减小梗死质量,减轻神经元损伤,对脑组织具有保护作用。

Objective To explore the protective effects of Zhenzhu Tongluo Pills on cerebral ischemia reperfusion injury in rats.Methods Totally 15 healthy SD rats were randomly divided into sham-operation group,model group and TCM group according to random number table method,with 5 rats in each group.Except for the sham-operation group,cerebral ischemia models in the other two groups were induced by temporary middle cerebral artery occlusion.After the establishment of ischemia models,the TCM group was administered Zhenzhu Tongluo Pills solutions(1 ml/100 g)intragastrically at a dose of 0.5 g/(kg·d),and the sham-operation group and model group were treated with normal saline,for 14 consecutive days.Neurological deficit score was used to evaluate the degree of neurological deficit in cerebral ischemia rats.TTC staining were used for determining the infarct weights.The nerve cell damage was observed using HE staining method.Immunohistochemistry staining was used to observe the expression levels of GFAP,Nestin,and NeuN.Results Compared with that at the 3 hours after reperfusion,the Bederson score in the TCM group decreased at the 30 minutes after the last administration(P<0.05);compared with the model group,the infarction ratio of rats in the TCM group decreased(P<0.05);the NeuN staining of neurons around the ischemic lesion in the model group rats was light,while Nestin and GFAP staining were deep;the Nestin and GFAP staining of neurons in the TCM group was light,while NeuN staining was deeper.Conclusion Zhenzhu Tongluo Pills can improve the symptom of neurological deficits,reduce the volume of infarction,and protect neurons from injury in rats with cerebral ischemia.