人参花蕾黄芪合剂对D-半乳糖模型雄性大鼠骨质疏松骨量的影响

Effect of the Compound of Ginseng Flower Bud And Astragalus on Osteoporosis Bone Mass in D-galactose Model Male Rats

目的观察人参花蕾黄芪合剂对雄性大鼠衰老时出现的原发性骨质疏松的预防作用。方法将3月龄60只雄性SD大鼠随机分为6组,对照组(NC组)、D-半乳糖模型组(DG组)、复方司坦唑醇(司坦唑醇+吡拉西坦)阳性对照组(SP组)、仙灵骨葆中药对照组(GB组)、人参花蕾黄芪合剂低剂量组(GFRAL组)及人参花蕾黄芪合剂高剂量组(GFRAH组),每组10只;连续给药3个月后,处死老鼠后取左胫骨进行骨形态计量学检测,取右股骨进行生物力学性能测定,取左股骨进行骨钙、磷酸、羟脯氨酸(Hyp)浓度测定。结果与NC组比较,DG组胫骨上段静态参数中骨小梁百分率(%Tb.Ar)、骨小梁数目(Tb.N)降低,骨小梁分离度(Tb.Sp)增加(P<0.05,P<0.01),胫骨上段动态参数单位骨小梁面积破骨细胞数(Oc.N)、破骨细胞周长百分数(%Oc.Pm)增加(P<0.01),矿化沉积率(MAR)、骨表面水平骨形成率(BFR/BS)、骨面积水平骨形成率(BFR/BV)、骨组织面积水平骨形成率(BFR/TV)下降(P<0.05),股骨Hyp下降(P<0.01)。与DG组比较,GFRAH、GFRAL组胫骨上段静态参数中的%Tb.Ar、Tb.Th、Tb.N增加(P<0.01,P<0.05),Tb.Sp减少(P<0.05);GFRAL组的股骨Hyp降低(P<0.05)。结论人参花蕾黄芪合剂与复方司坦唑醇和仙灵骨葆胶囊的预防效果相似,可有效预防D-半乳糖导致雄性大鼠的骨质疏松改变。

Objective To investigate the preventive effect of the compound of Ginseng flower bud and Astragalus on primary osteoporosis in aging male rats.Methods Sixty male SD rats,with 3 months old were randomly divided into 6 groups:the control group(NC group),D-galactose model group(DG group),positive control group(SP group,Stanazol+Piracetam),Chinese medicine control group(GB group,Xianling Gubao Capsule),the low-dose group of the compound of Ginseng Flower Bud and Astragalus(GFRAL group),and the high dose group of the compound of Ginseng Flower Bud and Astragalus(GFRAH group),10 in each group.After 3 months of continuous administration,all the rats were sacrificed.Then the left tibias were taken for bone histophiometry,the right femurs were used to detect biomechanical properties,and the left femurs were taken to measure bone calcium,phosphate and hydroxyproline(Hyp)concentrations.Results Compared with NC group,the static parameters of percent trabecular area(%Tb.Ar)and trabecular number(Tb.N)in upper tibia of DG group decreased,and the degree of trabecular spacing(Tb.Sp)increased(P<0.05,P<0.01).While osteoclasts number(Oc.N)and ostelclast perimeter/Tb.Pm×100(%Oc.Pm)per trabecular area of the dynamic parameters of upper tibia segment increased(P<0.01),mineral appositeion rate(MAR),bone formation rate of bone surface(BFR/BS),bone formation rate(BFR/BV),bone formation rate of trabecular volume(BFR/TV)decreased(P<0.05),and femoral Hyp decreased(P<0.01).Compared with DG group,the static parameters of%Tb.Ar,Tb.Th and Tb.N in upper tibia in GFRAH and GFRAL groups increased(P<0.01,P<0.05),and Tb.Sp decreased(P<0.05),respectively.The Hyp of femur in GFRAL group decreased(P<0.05).Conclusions The compound of Ginseng flower bud and Astragalus has similar preventive effect with compound Stanazol and Xianling Gubao Capsule.It can effectively prevent osteoporosis in male rats caused by D-galactose,and it is suitable for the elderly to use in daily life.could effectively prevent from osteoporosis caused by D-galactose in male aging rats,and the compound may have the potential to become an ideal anti-osteoporosis drug.