中国草原红牛不同部位肌纤维特性及类型

Characteristics and Types of Muscle Fibers in Different Parts of Chinese Red Steppe Cattle

为研究中国草原红牛不同部位肌纤维组织特性,对草原红牛不同部位肌纤维特性及类型进行比较分析。选择6头体质量、日龄相近的中国草原红牛,通过石蜡切片、苏木精-伊红(hematoxylin-eosin,HE)染色和烟酰胺腺嘌呤二核苷酸四唑氧化还原酶组织化学染色对不同部位肌肉样本(西冷、大条、米龙、上脑、小条及牛腩)进行显微观察并结合形态计量学分析。采取HE染色法对不同部位肌纤维直径、肌纤维横截面积、肌内膜厚度、肌束膜厚度及肌纤维类型组成占比进行测定和统计,同时进行差异性分析。结果表明:中国草原红牛不同部位间的肌纤维横截面积与肌纤维直径均不存在显著性差异;牛腩、西冷部位肌束膜厚度与其他部位差异极显著(P<0.01);西冷、大条肌内膜厚度与其他部位相比差异显著(P<0.05),Ⅰ型肌纤维占比各部位间均差异显著(P<0.05),ⅡB型肌纤维占比米龙、西冷部位与其他部位差异极显著(P<0.01)。结果提示,草原红牛不同部位间肌纤维组织特性及肌纤维组成占比存在差异。

A comparative analysis was conducted on the characteristics and types of muscle fibers in different parts of Chinese red steppe cattle.Six Chinese red steppe cattle with similar body mass and age were selected and slaughtered.Muscle samples from six different carcass parts including sirloin,outsideflat,outside,chuck roll,eye round andflank,were collected for observation under microscope and morphometric analysis through paraffin sectioning,hematoxylin-eosin(HE)staining,and nicotinamide adenine dinucleotide-tetrazolium reductase(NADH-TR)staining.The HE staining method was used to measure and statistically analyze the diameter,cross-sectional area,endomysium thickness,perimysium thickness,and proportion of different musclefiber types,and differential analysis was conducted.The results showed that there was no significant difference in the cross-sectional area or diameter of musclefibers between different parts of Chinese red steppe cattle.The perimysium thickness of brisket and sirloin muscles showed significant differences compared to those of the other muscles(P<0.01).The sirloin and outsideflat showed a significant difference in the endomysium thickness of muscle compared to the other parts(P<0.05).The proportion of type I musclefibers showed significant differences among different parts(P<0.05).The proportion of type IIB musclefibers in the outside and sirloin showed significant differences compared to the other parts(P<0.01).Thesefindings indicate that there are differences in the characteristics and proportion of musclefibers among different parts of Chinese red steppe cattle.