静电喷雾法一步制备自显影Fe_(3)O_(4)@壳聚糖载药栓塞微球

One-step preparation of self-imaging Fe_(3)O_(4)@chitosan drug-loaded embolic microspheresby electrostatic spraying

采用静电喷雾法一步制备包裹着Fe_(3)O_(4)纳米粒子的壳聚糖复合微球(Fe_(3)O_(4)@CS微球),实现Fe_(3)O_(4)纳米粒子与微球同时合成。还可以按需制备粒径范围为90~1 000μm的Fe_(3)O_(4)@CS微球,以满足不同部位血管的临床栓塞要求。SEM显示微球形貌均匀且粒径分布均一((94±3)μm),体外降解实验证明了微球具有生物可降解性,磁共振成像测试表明所制备的Fe_(3)O_(4)@CS微球具有良好的临床成像能力,血液、细胞相容性评估证实Fe_(3)O_(4)@CS微球具有良好的生物相容性。负载盐酸阿霉素(DOX)的载药微球显示出典型的药物缓释曲线,72 h内DOX的累计释放率为28.82%。结果表明,这一步可控制备的自显影栓塞剂在经导管动脉栓塞术(TACE)未来应用中展示了巨大的潜力。

Chitosan composite microspheres encapsulating Fe_(3)O_(4) nanoparticles(Fe_(3)O_(4)@CS microspheres)were prepared in one step by the electrostatic spraying,enabling simultaneous synthesis of Fe_(3)O_(4) nanoparticles and microspheres.Additionally,Fe_(3)O_(4)@CS microspheres with particle sizes ranging from 90 to 1000μm could be prepared on demand to meet the clinical embolization requirements of different blood vessels.SEM images showed that the microspheres were homogeneous in shape and uniform in size distribution((94±3)μm).In vitro degradation experiments proved that the microspheres were biodegradable.Magnetic resonance imaging showed that the prepared Fe_(3)O_(4)@CS microspheres had excellent clinical imaging capabilities.Moreover,blood and cytocompatibility assessments confirmed the good biocompatibility of Fe_(3)O_(4)@CS microspheres.The drug-loaded microspheres loaded with doxorubicin hydrochloride(DOX)showed a typical drug sustained release curve,and the cumulative release rate of DOX was 28.82%within 72 h.These results demonstrate that one-step controllable preparation of selfimaging embolic agents show great potential for future applications in transcatheter arterial embolization(TACE).