取食施硅水稻后白背飞虱体内解毒酶和保护酶活性的变化

Changes in the activities of detoxifying and protective enzymes in Sogatella furcifera(Hemiptera∶Delphacidae)adults fed on rice plants amended with silicon

为探究取食施硅水稻后白背飞虱Sogatella furcifera体内解毒酶(羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GST)、多功能氧化酶(MFO))和保护酶(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD))活性的变化。以感虫品种TN1为供试水稻品种,采用营养液添加硅酸钠(Na_(2)SiO_(3)·9H_(2)O)的方法设置施硅(Si+, 112 mg Si/L)和不施硅(Si-, 0 mg Si/L)处理,测定取食Si+或Si-水稻24 h、48 h、72 h和96 h后白背飞虱成虫体内CarE、GST、MFO、SOD、CAT和POD活性的动态变化。结果显示,与取食Si-水稻的白背飞虱相比,取食Si+水稻48 h、72 h和96 h时,CarE活性显著降低,GST活性显著升高;取食72 h和96 h时,MFO活性显著上升。取食Si+水稻24 h和48 h时,SOD和CAT活性显著增大;取食72 h时,SOD和POD活性显著增高;取食96 h时,3种保护酶活性均降低,其中SOD和POD活性显著降低。以上结果表明,施硅可能通过影响白背飞虱体内解毒酶和保护酶活性的动态平衡,进而对白背飞虱生理代谢造成影响。本研究为合理利用硅肥防治白背飞虱提供了理论依据。

In order to investigate the changes of detoxifying and protective enzymes in Sogatella furcifera adults fed on rice plants amended with silicon(Si).A susceptible rice variety Taichung Native 1(TN1)was used.S.furcifera adults were fed on leaf sheaths of TN1 rice plants grown in nutrient solutions added with 112 mg Si/L sodium silicate(Na_(2)SiO_(3)·9H_(2)O)(Si+)or fed on aquacultured plants without addition of sodium silicate(Si-).Activities of carboxylesterase(CarE),glutathione-S-transferases(GST),mixed-functional oxidase(MFO),superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)were measured in the Si+and Si-adults fed for 24 h,48 h,72 h,and 96 h on the plants.The results showed that Si addition generally decreased CarE activity but increased GST and MFO activities.CarE activity were significantly lower in the Si+than in the Si-adults fed for 48,72 h and 96 h,whereas GST activity increased significantly at 48,72 and 96 h.MFO activity increased significantly at 72 h and 96 h.SOD and CAT activities were significantly higher in the Si+than in the Si-adults fed for 24 h and 48 h,SOD and POD activities were significantly higher in the Si+than in the Si-adults fed for 72 h.When the feeding duration was elongated to 96 h,the activities of three protective enzymes declined in Si+adults in comparison with those in Si-adults,SOD and POD activities declined significantly.These results indicated that Si amendment to rice plants might influence the activities of detoxifying and protective enzymes in S.furcifera,which in return affected the physiological metabolism of S.furcifera.This study provides a theoretical basis for insect pest management by using silicon fertilizer in rice production.