海南省某奶牛场“两病”净化效果监测分析

Monitoring of the Eradication Effectiveness of Brucellosis and Tuberculosis in A Dairy Farm in Hainan Province

【目的】海南省某奶牛场为建设全省首个布鲁氏菌病和牛结核病(两病)净化场,2020-2023年连续四年对该场进行监测验证。【方法】参照国家颁布的《动物布鲁氏菌病诊断技术》(GB/T 18646-2018)和《动物结核病诊断技术》(GB/T 18645-2020)标准,采用虎红平板凝集试验(RBT)、试管凝集实验(SAT)、牛结核菌素皮内变态反应(PDD)等方法对牛群进行检测分析。【结果】总计采集布病检测样品2 104份,牛结核PDD试验2 063头。RBT初检阳性4年总计73头,平均个体阳性率3.5%,而SAT确诊4年阳性总计0份,阳性率为0,表明该奶牛场未患有布病;且2020-2023年RBT阳性率从5.7%逐年下降到1.8%,表明该奶牛场的净化成效显著。牛结核病检测结果显示,PDD疑似阳性4年总计80头,而60 d后复检阳性为0头,阳性率为0,表明该牛场为未患牛结核病牛场。【结论】2020-2023年海南该奶牛场针对“两病”净化工作成效明显,已进入净化维持阶段,初步建成海南省首个奶牛“两病”净化场。未来将继续提高疫病防控力度,以保障当地奶牛业稳定发展。

【Objective】A dairy farm in Hainan Province embarked on a pioneering mission to become the first farm to be declared free from both brucellosis and bovine tuberculosis in the region.The farm underwent comprehensive monitoring and verification from 2020 to 2023 to achieve this status.【Methods】Diagnostic testing followed national standards,namely“Diagnostic Techniques for Animal Brucellosis”(GB/T 18646-2018)and“Diagnostic Techniques for Animal Tuberculosis”(GB/T 18645-2020).Detection and analysis were performed using a combination of the Rose Bengal Plate Test(RBPT),Serum Agglutination Test(SAT),and Bovine Tuberculin PPD Skin Test,among other methods.【Results】Throughout the four-year period,a total of 2,104 brucellosis tests and 2,063 cattle tuberculosis PPD tests were conducted.Of those tested for brucellosis via RBPT,73 cows returned positive results,yielding an average individual positivity rate of 3.5%.Notably,the SAT diagnosis over the same period concluded with a zero positivity rate,indicating the absence of active brucellosis on the farm.Furthermore,the gradual decrease in the RBPT positivity rate from 5.7%to 1.8%exemplifies the substantial progress in eradicating brucellosis.Bovine tuberculosis detection revealed 80 suspected positive responses in PPD tests,but none retested positive after 60 days,ultimately demonstrating a non-existent infection rate.【Conclusion】The comprehensive disease eradication strategy carried out from 2020 to 2023 on the Hainan dairy farm yielded significant results,propelling the farm into a maintenance phase of disease-free status.The establishment of the first dairy farm in Hainan Province free from both brucellosis and bovine tuberculosis is an important milestone and serves as a testament to the effectiveness of the implemented preventive measures.Continued efforts in disease prevention and control will be crucial to safeguard the continued progress and sustainability of the local dairy industry.