氚水对斑马鱼早期发育阶段的剂量-效应关系研究

Study on the dose-effect relationship of tritium water in the early growth and development of zebrafish

目的研究氚水对早期发育阶段的斑马鱼造成的辐射剂量, 并与其造成的生物效应相结合, 初步观察剂量-效应关系。方法模拟真实实验条件, 建立物理模型, 计算斑马鱼受精后24 h (24 hpf)胚胎和96 hpf幼鱼在3.7×10^(3)、3.7×10^(4)、3.7×10^(5) Bq/ml 3种不同浓度氚水中的吸收剂量率。观察经3种不同浓度氚水染毒后24 hpf胚胎和96 hpf幼鱼的翻转频率和心率, 并与对照组(E3溶液)进行比较。多组间比较采用单因素方差分析, 对照组与处理组之间的比较采用LSD-t检验。结果随着斑马鱼发育时间的延长, 其吸收剂量有所提高。24 hpf斑马鱼胚胎在3种不同浓度的氚水中对应的吸收剂量率分别为2.15×10、2.21×10^(2)、2.55×10^(3) μGy/h;96 hpf斑马鱼幼鱼对应的吸收剂量率分别为2.95×10、3.03×10^(2)、3.47×10^(3) μGy/h。24 hpf斑马鱼胚胎翻转实验结果显示, 与对照组比较, 3.7×10^(3) Bq/ml氚水处理组的翻转频率明显减少(t=3.94, P<0.001)。96 hpf斑马鱼幼鱼心率随着不同氚水浓度的变化明显, 与对照组比较, 3.7×10^(3) Bq/ml氚水处理组心率明显下降(t=2.86, P=0.01), 而3.7×10^(5) Bq/mL氚水处理组心率上升(t=-12.12, P<0.001)。结论随着吸收剂量率的变化, 24 hpf斑马鱼胚胎翻转频率和96 hpf斑马鱼幼鱼心率均出现显著改变。

Objective To study the effect of tritium water radiation dose on zebrafish at early developmental stages and integration with biological effects for the preliminary observation of dose–effect relationships.Methods The real experimental conditions were simulated,the physical conditions were modeled,and the absorbed dose rates were calculated for 24 hours post fertilization(hpf)embryos and 96 hpf larvae in three different concentrations of tritium water:3.7×10^(3),3.7×10^(4),and 3.7×10^(5) Bq/ml.The embryos or larvae turnover frequency and heart rate of 24 and 96 hpf were observed and compared with those of the control group.Comparisons between multiple groups were analyzed by one-way ANOVA,and differences between the control and treatment groups were compared for significance using the LSD-t test.Results With the extension of development time,the absorbed dose of zebrafish increased in three different concentrations of tritiated water.24 hpf embryos corresponding to dose rates of 2.15×10,2.21×10^(2),2.55×10^(3)μGy/h;96 hpf larvae corresponding to dose rates of 2.95×10,3.03×10^(2),3.47×10^(3)μGy/h.Comparison with control group,the results of 24 hpf embryo flipping showed that the significantly fewer embryos turned over in the tritium water-stained group whith 3.7×10^(3) Bq/ml(t=3.94,P<0.001);and 96 hpf larvae heart rate changes significantly with tritium water concentration.Comparison with control group,the 3.7×10^(3) Bq/ml tritium water-treated group had a significant decrease in heart rate(t=2.86,P=0.01),while the 3.7×10^(5) Bq/ml tritium water-treated group had an increase in heart rate(t=-12.12,P<0.001).Conclusion Significant changes in embryonic turnover at 24 hpf and heart rate of larvae at 96 hpf were observed with changes in absorbed dose rate.