结肠癌患者miR-148a-3p、miR-19a表达及与预后的关系

Expressions of miR-148a-3p and miR-19a in patients with colon cancer and their relation-ship with prognosis

目的探究结肠癌患者miR-148a-3p、miR-19a表达及其与预后的关系。方法选择2019年1月至2020年1月于郑州大学人民医院就诊的98例结肠癌患者作为观察组,并选择同期于本院进行体检且结果为健康者49例作为对照组。收集所有研究对象入组时的一般资料;比较两组患者血清miR-148a-3p、miR-19a表达量;比较不同临床特征患者的miR-148a-3p、miR-19a表达;随访患者生存时间,采用单因素生存分析Kaplan-Meier绘制生存曲线,COX风险比例回归分析影响患者预后的因素。结果观察组血清miR-148a-3p表达量显著低于对照组,差异有统计学意义(t=2.669,P<0.05),miR-19a的表达量显著高于对照组,差异有统计学意义(t=2.929,P<0.05);结肠癌患者miR-148a-3p、miR-19a表达水平与肿瘤分化程度、TNM分期有关(t=4.075、2.110,P<0.05);miR-148a-3p低表达患者3年总生存率和3年无病生存率均显著低于高表达患者(χ^(2)=6.978,8.025,P<0.05);miR-19a高表达患者3年总生存率和3年无病生存率均显著低于低表达患者(χ^(2)=8.252,4.474,P<0.05);miR-148a-3p下调、miR-19a上调为影响结肠癌患者3年无病生存率的独立危险因素(P<0.05)。结论结肠癌患者血清miR-148a-3p异常低表达,miR-19a异常高表达,且其异常表达可降低患者3年总生存率和无病生存率。

Objective To explore the expressions of microRNA(miR)-148a-3p and miR-19a,and their relationship with prognosis in patients with colon cancer.Methods A total of 98 patients with colon cancer and 49 healthy controls in People's Hospital of Zhengzhou University were enrolled as the observation group and the control group from January 2019 to January 2020,respectively.The general data of all objects were collected and enrollment.The expression levels of serum miR-148a-3p and miR-19a were compared between the two groups and among patients with different clinical characteristics.The survival time of patients was followed up.The survival curves were drawn by Kaplan-Meier analysis.The influencing factors of prognosis were analyzed by COX proportional hazard regression.Results The expression level of serum miR-148a-3p in the observation group was significantly lower than that in the control group(t=2.669,P<0.05),while miR-19a was significantly higher than that in the control group(t=2.929,P<0.05).The expression levels of miR-148a-3p and miR-19a were correlated with the degree of tumor differentiation and TNM stage(t=4.075,2.110,P<0.05).The 3-year overall survival rate and 3-year disease-free survival rate in patients with low ex-pression of miR-148a-3p were significantly lower than those with high expression(χ^(2)=6.978,8.025,P<0.05).The 3-year overall survival rate and disease-free survival rate in patients with high expression of miR-19a were significantly lower than those with low expression(χ^(2)=8.252,4.474,P<0.05).Downregulated of miR-148a-3p and upregulated of miR-19a were independent risk factors affecting the 3-year disease-free survival rate of patients with colon cancer(OR=0.024,2.642,P<0.05).Conclusion Patients with colon cancer have abnor-mally low expression of serum miR-148a-3p and abnormally high expression of miR-19a,and their abnormal expression can reduce the patient's 3-year overall survival rate and disease-free survival rate.