血浆miR-379、miR-195、Gas6水平早期检测在AMI患者中的应用价值

Application value of early detection of plasma miR-379,miR-195 and Gas6 levels in patients with AMI

目的分析血浆微小RNA-379(miR-379)、微小RNA-195(miR-195)、生长停滞特异性蛋白6(Gas6)水平早期检测在急性心肌梗死(AMI)患者中的应用价值。方法选取2021年5至2022年7月于首都医科大学附属北京世纪坛医院心内科住院治疗的265例患者,分为AMI组(n=127)和非AMI组(n=138),选取同期120名健康体检者设为对照组。比较三组入组时的血浆miR-379、miR-195表达量及Gas6水平;采用Pearson相关系数分析AMI患者入组时的血浆Gas6水平与miR-379、miR-195表达量的关系;采用受试者工作曲线(ROC)分析入组时的血浆Gas6水平及miR-379、miR-195表达量对早期AMI的诊断价值。结果三组入组时的血浆miR-379、miR-195、Gas6水平比较差异有统计学意义(P<0.05);血浆miR-379水平比较:AMI组<非AMI组<对照组,miR-195、Gas6水平比较:AMI组>非AMI组>对照组,差异有统计学意义(P<0.05)。Pearson相关系数分析显示,Gas6与miR-379水平呈负相关(P<0.05),与miR-195水平呈正相关(P<0.05)。ROC结果显示,血浆miR-379、miR-195、Gas6水平单独诊断早期AMI具有一定局限性(AUC=0.808、0.718、0.752),三者联合诊断的效能更佳(AUC=0.879)。结论血浆miR-379、miR-195、Gas6水平在AMI患者中异常表达,三指标可作为AMI早期疾病诊断的参考指标。

Objective To analyze the application value of early detection of plasma microRNA-379(miR-379),microRNA-195(miR-195)and growth arrest specific protein 6(Gas6)levels in patients with acute myocardial infarction(AMI).Methods 265 patients who were hospitalized in the Department of Cardiology,Beijing Shijitan Hospital Affiliated to Capital Medical University were selected between May 2021 and July 2022,and were classified into the AMI group(n=127)and the non-AMI group(n=138).120 healthy subjects with physical examination during the same period were selected as the control group.The expression levels of plasma miR-379 and miR-195 and Gas6 at admission were compared among the three groups.Pearson correlation coefficient analysis was used to analyze the relationship between plasma Gas6 level and expression levels of miR-379 and miR-195 in patients with AMI at admission.The diagnostic value of plasma Gas6 level and expression levels of miR-379 and miR-195 at admission on early AMI was analyzed by the receiver operating characteristic curve(ROC).Results There were statistically significant differences in the plasma levels of miR-379,miR-195,and Gas6 among the three groups at enrollment(P<0.05).Comparison of plasma miR-379 levels:AMI group<non-AMI group<control group(P<0.05).Comparison of miR-195 and Gas6 levels:AMI group>non-AMI group>control group(P<0.05).Pearson correlation coefficient analysis showed that Gas6 was negatively correlated with the level of miR-379(P<0.05)and positively correlated with the level of miR-195(P<0.05).The ROC results showed that plasma miR-379,miR-195 and Gas6 alone had certain limitations in diagnosing early AMI(AUC=0.808,0.718 and 0.752),and the combination of the three had better efficiency(AUC=0.879).Conclusion The levels of plasma miR-379,miR-195 and Gas6 are abnormally expressed in patients with AMI,and these three indicators can be used as reference indicators for the early diagnosis of AMI.