2型糖尿病肾病患者血清NF-κB炎症信号通路与肾功能指标的相关性

Correlation between Serum NF-κB Inflammatory Signaling Pathway and Renal Function Indicators in Patients with Type 2 Diabetic Nephropathy

【目的】探讨2型糖尿病肾病(T2DN)患者血清核转录因子-κB(NF-κB)炎症信号通路与肾功能指标[血尿素氮(BUN)、血肌酐(Scr)、尿白蛋白排泄率(UAER)、β2-微球蛋白(β2-MG)、肾小球滤过率(eGFR)]的相关性。【方法】初诊的T2DN患者87例为A组,同期收治的单纯原发性2型糖尿病患者80例为B组,体检健康的志愿者70例为C组。比较三组临床资料,肾功能指标及血清NF-κB、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、高敏C反应蛋白(hs-CRP)水平。采用Pearson相关法分析T2DN患者血清NF-κB、TNF-α、IL-1β、hs-CRP、IL-6水平与肾功能指标的相关性。【结果】A组和B组空腹血糖、餐后2 h血糖高于C组(P<0.05);三组其他临床资料比较,差异无统计学意义(P>0.05)。A组BUN、Scr、UAER、β2-MG高于B组和C组(P<0.05),eGFR低于B组和C组(P<0.05);B组与C组上述指标比较,差异无统计学意义(P>0.05)。A组血清NF-κB、TNF-α、IL-1β、IL-6、hs-CRP水平高于B组和C组(P<0.05);B组上述指标均高于C组(P<0.05)。Pearson相关分析显示,T2DN患者血清NF-κB、TNF-α、IL-1β、IL-6、hs-CRP水平与BUN、Scr、UAER、β2-MG均呈正相关(P<0.05),与eGFR均呈负相关(P<0.05)。【结论】NF-κB炎症信号通路的激活介导了T2DN的发生、发展,且NF-κB、TNF-α、IL-1β、IL-6、hs-CRP均与肾功能指标存在明显相关性。

【Objective】To investigate the correlation between serum nuclear factor-kappa B(NF-κB)inflammatory signaling pathway and renal function indicators such as blood urea nitrogen(BUN),serum creatinine(Scr),urinary albumin excretion rate(UAER),β2-microglobulin(β2-MG),and estimated glomerular filtration rate(eGFR)in patients with type 2 diabetic nephropathy(T2DN).【Methods】Eighty-seven newly diagnosed T2DN patients(Group A),eighty patients with primary type 2 diabetes mellitus without nephropathy(Group B),and seventy healthy volunteers(Group C)were enrolled in the study.Clinical data,renal function indicators,and serum levels of NF-κB,tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and high-sensitivity C-reactive protein(hs-CRP)were compared among the three groups.Pearson correlation analysis was used to analyze the relationship of renal function indicators with serum levels of NF-κB,TNF-α,IL-1β,IL-6 and hs-CRP,in T2DN patients.【Results】Fasting blood glucose and 2-hour postprandial blood glucose in both group A and group B were higher than those in group C(P<0.05).Other clinical data showed no significant difference among the three groups(P>0.05).Levels of BUN,Scr,UAER,andβ2-MG in group A were higher than those in groups B and C(P<0.05),while level of eGFR in group A was lower than that in groups of B and C(P<0.05).There were no significant differences in these above indicators between group B and group C(P>0.05).Serum levels of NF-κB,TNF-α,IL-1β,IL-6,and hs-CRP in group A were higher than those in Groups B and C(P<0.05);The above indicators of group B also were higher than those of group C(P<0.05).Pearson correlation analysis showed that serum levels of NF-κB,TNF-α,IL-1β,IL-6,and hs-CRP in T2DN patients were positively correlated with levels of BUN,Scr,UAER,andβ2-MG(P<0.05),while they were negatively correlated with eGFR level(P<0.05).【Conclusion】Activation of the NF-κB inflammatory signaling pathway mediates the pathogenesis of T2DN.NF-κB,TNF-α,IL-1β,IL-6 and hs-CRP are significantly correlated with renal function indicators.