摘要
目的探讨右美托咪定对小鼠脑出血后神经功能损伤及肥大细胞激活的影响。方法小鼠脑出血前30 min经腹腔注射右美托咪定,脑出血模型制备完成后检测小鼠神经功能、脑含水量、血肿周围肥大细胞数量及类胰蛋白酶、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达量。结果与对照组比较,脑出血组的小鼠神经系统功能评分明显提升(P<0.05),脑含水量明显增加(P<0.05),肥大细胞数量明显增加(P<0.05),类胰蛋白酶、IL-1β和TNF-α表达量明显增加(P<0.05);而右美托咪定组的小鼠神经功能评分明显下降(P<0.05),脑含水量明显减少(P<0.05),肥大细胞数量明显减少(P<0.05),类胰蛋白酶、IL-1β和TNF-α表达量明显减少(P<0.05)。结论右美托咪定可以抑制脑出血后血肿周边肥大细胞的激活,减轻小鼠神经功能损害。
Objective To explore the effect of dexmedetomidine on the neurological function and mast cells activation in the mouse with intracerebral hemorrhage(ICH).Methods The mouse was intraperitoneally injected with dexmedetomidine at 30 min before intracerebral hemorrhage.After the preparation of intracerebral hemorrhage model,the neurological function,brain water content,number of mast cells around hematoma and expression levels of tryptase,IL-1β,TNF-αwere detected.Results Compared with the control group,the neurological function score in the intracerebral hemorrhage group was significantly elevated(P<0.05),the brain water content was significantly increased(P<0.05),the mast cells number was significantly increased(P<0.05),and the tryptase,IL-1βand TNF-αexpression levels were sinificantly increased(P<0.05);while the neurological function score in the dexmedetomidine group was significantly decreased(P<0.05),the brain water content was significantly decreased(P<0.05),the number of mast cells was significantly reduced and the tryptase,IL-1βand TNF-αexpression levels were significantly decreased(P<0.05).Conclusion Dexmedetomidine could inhibit the activation of mast cells around hematoma and reduce the damage of neurological function after mouse intracerebral hemorrhage.
作者
杨正宇
毛庆祥
YANG Zhengyu;MAO Qingxiang(Department of Anesthesiology,Daping Hospital,Army Military Medical University,Chongqing 400042,China)
出处
《重庆医学》
CAS
2024年第2期171-175,共5页
Chongqing medicine
基金
重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0018)。
关键词
右美托咪定
脑出血
肥大细胞
神经功能
dexmedetomidine
intracerebral hemorrhage
mast cell
neurological function