一锅水热法合成磷掺杂碳点用于芦丁的检测

One-Pot Hydrothermal Synthesis of Phosphorus-Doped Carbon Dots for the Determination of Rutin

芦丁常用于防治脑溢血、高血压、视网膜出血、紫癜和急性出血性肾炎等疾病,因此迫切需要开发高效快速检测芦丁的方法。以4,4’-联吡啶为碳源和氮源、磷酸为磷源,采用一锅水热法制备得到水溶性好、选择性强、绿色、低毒的碳点。通过透射电子显微镜(TEM)、傅里叶变换红外(FT-IR)光谱仪、X射线衍射仪(XRD)、X射线光电子能谱仪(XPS)、紫外-可见(UV-Vis)光谱仪和荧光光谱仪表征了该磷掺杂碳点(P-CD)的粒径、官能团种类、元素组成及光学性质。该P-CD的最大激发波长为390 nm,最大发射波长为516 nm;碳点整体呈球形,且粒径小于10 nm。基于芦丁对P-CD荧光的猝灭现象,构建了高选择性与高灵敏度的荧光碳点检测芦丁的方法。结果表明,在芦丁浓度为10~100μmol·L^(-1)时,芦丁浓度与P-CD的荧光强度之间具有良好的线性关系,线性相关系数(R^(2))为0.9960。

Rutin is often used to prevent and control cerebral hemorrhage,hypertension,retinal hemorrhage,purpura and acute hemorrhagic nephritis,etc.,therefore it is urgent to develop efficient and rapid methods for the detection of rutin.With 4,4'-bipyridine as carbon and nitrogen sources and phosphoric acid as phosphorus source,carbon dots with good water solubility,strong selectivity,green and low toxicity were prepared by one-pot hydrothermal method.The particle size,functional group type,elemental composition and optical properties of the phosphorus-doped carbon dots(P-CDs)were characterized by transmission electron microscope(TEM),Fourier transform infrared(FT-IR)spectrometer,X-ray diffractometer(XRD),X-ray photoelectron spectrometer(XPS),ultraviolet-visible(UV-Vis)spectrometer and fluorescence spectrophotometer.The maximum excitation wavelength of the P-CDs is 390 nm,and the maximum emission wavelength is 516 nm.The carbon dots are spherical in shape,and the particle size is less than 10 nm.Based on the quenching phenomenon of rutin on the fluorescence of the P-CDs,the method of detecting rutin by fluorescent carbon dots with high selectivity and high sensitivity was constructed.The results show that there is a good linear relationship between the concentration of rutin and the fluorescence quenching degree of P-CDs when the concentration of rutin is 10-100μmol·L^(-1),and the linear correlation coefficient(R^(2))is 0.9960.