不同核壳结构的CsPbBr_(3)纳米晶体的稳定性、生物相容性能

Stability and Biocompatibility of CsPbBr_(3)Nanocrystals with Different Core-Shell Structures

CsPbBr_(3)钙钛矿纳米晶体(PNC)由于出色的光学性能使其在生物领域具有广泛的应用前景,但CsPbBr_(3)PNC的低稳定性及高毒性大大限制了其实际应用。因此,通过壳层包裹,采用室温重结晶法分别将环糊精(CD)和SiO_(2)包覆在CsPbBr_(3)PNC表面合成核壳结构的CsPbBr_(3)@CD和CsPbBr_(3)@SiO_(2)。不同的包覆材料使PNC具有不同的光学特性、稳定性及生物相容性。其中,CsPbBr_(3)@SiO_(2)的荧光量子产率(36.16%)约为CsPbBr_(3)@CD(14.39%)的2.5倍。CsPbBr_(3)@SiO_(2)的平均荧光衰减寿命(101.43 ns)约为CsPbBr_(3)@CD(68.73 ns)的1.5倍。在30℃、湿度为60%的空气中放置10天后,CsPbBr_(3)@SiO_(2)荧光强度约为CsPbBr_(3)@CD的3.9倍。将CsPbBr_(3)@CD和CsPbBr_(3)@SiO_(2)与细胞共培养,细胞存活率分别为51.27%和88.57%。结果表明,相较于CsPbBr_(3)@CD,CsPbBr_(3)@SiO_(2)具有更好的光学特性、稳定性以及生物相容性,更有利于在生物医学领域应用。

Due to excellent optical properties,CsPbBr_(3)perovskite nanocrystals(PNCs)have wide application prospects in the biological field,while the low stability and high toxicity of CsPbBr_(3)PNCs greatly limit their practical application.Therefore,CsPbBr_(3)@CD and CsPbBr_(3)@SiO_(2)with core-shell structures were synthesized by coating cyclodextrin(CD)and SiO_(2)on the surface of CsPbBr_(3)PNCs by shell coating and recrystallization at room temperature,respectively.Different coating materials make PNCs have different optical properties,stability and biocompatibility.The fluorescence quantum yield of CsPbBr_(3)@SiO_(2)(36.16%)is about 2.5 times that of CsPbBr_(3)@CD(14.39%).The mean fluorescence decay lifetime of CsPbBr_(3)@SiO_(2)(101.43 ns)is about 1.5 times that of CsPbBr_(3)@CD(68.73 ns).The fluorescence intensity of CsPbBr_(3)@SiO_(2)is about 3.9 times that of CsPbBr_(3)@CD after 10 days in air at 30℃and 60%humidity.CsPbBr_(3)@CD and CsPbBr_(3)@SiO_(2)were co-cultured with the cells.The cell survival rates of CsPbBr_(3)@CD and CsPbBr_(3)@SiO_(2)are 51.27%and 88.57%,respectively.The results show that compared with CsPbBr_(3)@CD,CsPbBr_(3)@SiO_(2)has better optical properties,stability and biocompatibility,which is more suitable for the applications in the biomedical field.