九肽-1/依克多因经皮共递送纳米脂质体美白修复功效研究

Study on whitening and repairing efficacy of nonapeptide-1/ectoin nanoliposomes

目的探讨九肽-1/依克多因经皮共递送纳米脂质体(Non/Ect-NLPs)的美白修复功效。方法通过细胞活性测试研究Non/Ect-NLPs安全性,观察黑素细胞对纳米脂质体摄取情况,并比较不同水平Non/Ect-NLPs组及游离成分溶液组酪氨酸酶活性、黑色素水平、氧化损伤细胞存活率及人源水通道蛋白-3(AQP3)、丝聚合蛋白(FLG)、紧密连接蛋白1(Claudin-1)水平。结果游离成分溶液组和不同水平Non/Ect-NLPs组中HaCaT细胞活性均在80%以上。与游离成分溶液组比较,Non/Ect-NLPs水平为250.0、500.0μg/mL时,其对B16F10细胞酪氨酸酶活性显著降低(P<0.05)。与游离成分溶液组比较,Non/Ect-NLPs水平为125.0、500.0μg/mL时,其能显著抑制B16F10细胞分泌黑色素(P<0.05)。与模型组比较,Non/Ect-NLPs水平为250.0、500.0μg/mL时,可显著提高氧化损伤细胞的存活率(P<0.05)。与对照组比较,游离成分溶液组和Non/Ect-NLPs组能显著促进HaCaT细胞迁移(P<0.01)。与对照组比较,游离成分溶液组、Non/Ect-NLPs组AQP3、FLG、Claudin-1水平显著增高(P<0.05)。结论Non/Ect-NLPs作为新型皮肤美白修复功效原料具有良好的应用前景。

Objective To explore the whitening and repairing effects of nonapeptide-1/ectoin nanoliposomes(Non/Ect-NLPs).Methods The safety of Non/Ect-NLPs was studied by cell viability test,and the uptake of nanoliposomes by melanocytes was observed.Tyrosinase activity,melanin levels,survival rate of oxidative damage cells,and the levels of human aquaporin-3(AQP3),filaggrin(FLG),and tight junction protein-1(Claudin-1)levels were compared between groups with different levels of Non/Ect-NLPs and free component solution groups.Results The viability of HaCaT cells in both the free component solution group and the groups with different levels of Non/Ect-NLPs were all above 80%.Compared with the free component solution group,when the levels of Non/Ect-NLPs were 250.0 and 500.0μg/mL,the tyrosinase activity of B16F10 cells was significantly decreased(P<0.05).Compared with the free component solution group,when the levels of Non/Ect-NLPs were 125.0 and 500.0μg/mL,it could significantly inhibit the secretion of melanin by B16F10 cells(P<0.05).Compared with the model group,Non/Ect-NLPs at 250.0 and 500.0μg/mL significantly increased the survival rate of oxidative damaged cells(P<0.05).Compared with the control group,the free component solution group and the Non/Ect-NLPs group significantly promoted the migration of HaCaT cells(P<0.01).Compared with the control group,the free component solution group and the Non/Ect-NLPs group had significant increases in AQP3,FLG,and Claudin-1 levels(P<0.05).Conclusion Non/Ect-NLPs have a good application prospect as a new type of skin whitening and repair efficacy raw material.