理肺散提取物乙酸乙酯层的化学成分及其对RGC-5细胞损伤的保护作用研究

Chemical Constituents of Ethyl Acetate Layer Extracted from Hedyotis Scandens and Its Protective Effect on RGC-5 Cell Injury

目的分析理肺散提取物乙酸乙酯层的化学成分,并探讨其对RGC-5细胞损伤的保护作用。方法采用硅胶柱、MCI柱等柱色谱技术对理肺散提取物乙酸乙酯层进行分离,采用制备型高效液相色谱进行纯化,利用核磁共振法对化合物进行结构鉴定,采用CCK-8法检测化合物对丙泊酚诱导的RGC-5细胞损伤的保护作用。结果共分离鉴定出15个化合物,分别为capsaicin(化合物1)、homodihydrocapsaicinⅠ(化合物2)、secoisolariciresinol(化合物3)、lariciresinol(化合物4)、dehydrodiconiferyl alcohol(化合物5)、cleomiscosin A(化合物6)、curcasinlignan B(化合物7)、syringaresinol(化合物8)、oleanolic acid(化合物9)、ursolic acid(化合物10)、methyl 4-benzoyloxy-3-methoxybenzeneacetate(化合物11)、(R)-6-methyl-4,6-bis(4-methylpent-3-enyl)cyclohexa-1,3-dienecarbaldehyde(化合物12)、2-hydroxy-1-methoxy-anthraquinone(化合物13)、isoscopoletin(化合物14)、1H-indole-3-carboxaldehyde(化合物15)。化合物1,4,6-8对丙泊酚诱导的RGC-5细胞损伤具有一定保护作用,其中化合物4、化合物7活性最强,分别使RGC-5细胞的活力提升了15.89%和13.09%。结论化合物1-7为首次从耳草属中分离发现,化合物9-10,13为首次从理肺散中分离发现。化合物4、化合物7对丙泊酚诱导的RGC-5细胞损伤显示出较好的保护作用。

Objective To analyze the chemical constituents of the ethyl acetate layer extracted from Hedyotis scandens,and to investigate its protective effect on RGC-5 cell injury.Methods The ethyl acetate layer extracted from Hedyotis scandens was isolated by the silica gel column,and MCI column,and it was purified by the preparative high-performance liquid chromatography(HPLC).The structure of the compound was identified by Magnetic Resonance Imaging(MRI)method,and the protective effect of the compound on propofol-induced RGC-5 cell injury was detected by the CCK-8 method.Results Fifteen compounds were isolated and identified,including capsaicin(compound 1),homodihydrocapsaicinⅠ(compound 2),secoisolariciresinol(compound 3),lariciresinol(compound 4),dehydrodiconiferyl alcohol(compound 5),cleomiscosin A(compound 6),curcasinlignan B(compound 7),syringaresinol(compound 8),oleanolic acid(compound 9),ursolic acid(compound 10),methyl 4-benzoyloxy-3-methoxybenzeneacetate(compound 11),(R)-6-methyl-4,6-bis(4-methylpent-3-enyl)cyclohexa-1,3-dienecarbaldehyde(compound 12),2-hydroxy-1-methoxy-anthraquinone(compound 13),isoscopoletin(compound 14),1H-indole-3-carboxaldehyde(compound 15).Compounds 1,4,6-8 had certain protective effects on the propofol-induced RGC-5 cell injury,among which compounds 4 and 7 had the most significant activity,and the cell viability was increased by 15.89%and 13.09%,respectively.Conclusion Compounds 1-7 were isolated and discovered for the first time from the genus Hedyotis,while compounds 9-10 and 13 were isolated and discovered for the first time from Hedyotis scandens.Compounds 4 and 7 showed good protective effects on the propofol-induced RGC-5 cell injury.